{"pmid":1,"file_date_updated":"2024-09-16T07:38:38Z","publisher":"Cell Press","type":"journal_article","citation":{"ista":"Hernandez-Armendariz A, Sorichetti V, Hayashi Y, Koskova Z, Brunner A, Ellenberg J, Šarić A, Cuylen-Haering S. 2024. A liquid-like coat mediates chromosome clustering during mitotic exit. Molecular Cell. 84(17), P3254–3270.E9.","ieee":"A. Hernandez-Armendariz <i>et al.</i>, “A liquid-like coat mediates chromosome clustering during mitotic exit,” <i>Molecular Cell</i>, vol. 84, no. 17. Cell Press, p. P3254–3270.E9, 2024.","chicago":"Hernandez-Armendariz, Alberto, Valerio Sorichetti, Yuki Hayashi, Zuzana Koskova, Andreas Brunner, Jan Ellenberg, Anđela Šarić, and Sara Cuylen-Haering. “A Liquid-like Coat Mediates Chromosome Clustering during Mitotic Exit.” <i>Molecular Cell</i>. Cell Press, 2024. <a href=\"https://doi.org/10.1016/j.molcel.2024.07.022\">https://doi.org/10.1016/j.molcel.2024.07.022</a>.","short":"A. Hernandez-Armendariz, V. Sorichetti, Y. Hayashi, Z. Koskova, A. Brunner, J. Ellenberg, A. Šarić, S. Cuylen-Haering, Molecular Cell 84 (2024) P3254–3270.E9.","ama":"Hernandez-Armendariz A, Sorichetti V, Hayashi Y, et al. A liquid-like coat mediates chromosome clustering during mitotic exit. <i>Molecular Cell</i>. 2024;84(17):P3254-3270.E9. doi:<a href=\"https://doi.org/10.1016/j.molcel.2024.07.022\">10.1016/j.molcel.2024.07.022</a>","apa":"Hernandez-Armendariz, A., Sorichetti, V., Hayashi, Y., Koskova, Z., Brunner, A., Ellenberg, J., … Cuylen-Haering, S. (2024). A liquid-like coat mediates chromosome clustering during mitotic exit. <i>Molecular Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molcel.2024.07.022\">https://doi.org/10.1016/j.molcel.2024.07.022</a>","mla":"Hernandez-Armendariz, Alberto, et al. “A Liquid-like Coat Mediates Chromosome Clustering during Mitotic Exit.” <i>Molecular Cell</i>, vol. 84, no. 17, Cell Press, 2024, p. P3254–3270.E9, doi:<a href=\"https://doi.org/10.1016/j.molcel.2024.07.022\">10.1016/j.molcel.2024.07.022</a>."},"date_updated":"2024-09-16T07:43:07Z","oa_version":"Published Version","acknowledgement":"We thank Daniel W. Gerlich for providing cell lines, the EMBL Advanced Light Microscopy Facility (ALMF) for support, Christian H. Haering and Thomas Quail for input on the manuscript, and Martina Dees for cloning several Ki-67 constructs. This work was supported by the German Research Foundation (DFG project number 402723784) and the Human Frontier Science Program (CDA00045/2019). A.H.-A. and A.B. have received PhD fellowships from the Boehringer Ingelheim Fonds, V.S. and A.Š. were supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant no. 802960), and Y.H. was supported by a fellowship from the EMBL interdisciplinary Postdoc (EIPOD) program (Marie Sklodowska-Curie Actions, COFUND grant agreement 664726).","date_created":"2024-09-15T22:01:41Z","ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"P3254-3270.E9","author":[{"last_name":"Hernandez-Armendariz","first_name":"Alberto","full_name":"Hernandez-Armendariz, Alberto"},{"first_name":"Valerio","full_name":"Sorichetti, Valerio","orcid":"0000-0002-9645-6576","last_name":"Sorichetti","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b"},{"full_name":"Hayashi, Yuki","first_name":"Yuki","last_name":"Hayashi"},{"last_name":"Koskova","full_name":"Koskova, Zuzana","first_name":"Zuzana"},{"last_name":"Brunner","full_name":"Brunner, Andreas","first_name":"Andreas"},{"first_name":"Jan","full_name":"Ellenberg, Jan","last_name":"Ellenberg"},{"last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","first_name":"Anđela","orcid":"0000-0002-7854-2139"},{"last_name":"Cuylen-Haering","first_name":"Sara","full_name":"Cuylen-Haering, Sara"}],"article_type":"original","date_published":"2024-09-05T00:00:00Z","language":[{"iso":"eng"}],"issue":"17","day":"05","status":"public","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","abstract":[{"lang":"eng","text":"The individualization of chromosomes during early mitosis and their clustering upon exit from cell division are two key transitions that ensure efficient segregation of eukaryotic chromosomes. Both processes are regulated by the surfactant-like protein Ki-67, but how Ki-67 achieves these diametric functions has remained unknown. Here, we report that Ki-67 radically switches from a chromosome repellent to a chromosome attractant during anaphase in human cells. We show that Ki-67 dephosphorylation during mitotic exit and the simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface. Experiments and coarse-grained simulations support a model in which the coalescence of chromosome surfaces, driven by co-condensation of Ki-67 and RNA, promotes clustering of chromosomes. Our study reveals how the switch of Ki-67 from a surfactant to a liquid-like condensed phase can generate mechanical forces during genome segregation that are required for re-establishing nuclear-cytoplasmic compartmentalization after mitosis."}],"publication_status":"published","external_id":{"pmid":["39153474"]},"intvolume":"        84","project":[{"grant_number":"802960","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","call_identifier":"H2020"}],"title":"A liquid-like coat mediates chromosome clustering during mitotic exit","file":[{"success":1,"content_type":"application/pdf","file_name":"2024_MolecularCell_HernandezArmendariz.pdf","date_updated":"2024-09-16T07:38:38Z","file_size":11654644,"access_level":"open_access","relation":"main_file","file_id":"18075","creator":"dernst","checksum":"3f360e0287b8ec79fb2b8b02b5070360","date_created":"2024-09-16T07:38:38Z"}],"ddc":["570"],"volume":84,"_id":"18072","department":[{"_id":"AnSa"}],"oa":1,"publication_identifier":{"eissn":["1097-4164"],"issn":["1097-2765"]},"year":"2024","publication":"Molecular Cell","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"doi":"10.1016/j.molcel.2024.07.022","quality_controlled":"1","month":"09","scopus_import":"1"}