---
res:
  bibo_abstract:
  - Conserved signaling cascades monitor protein-folding homeostasis to ensure proper
    cellular function. One of the evolutionary conserved key players is IRE1, which
    maintains endoplasmic reticulum (ER) homeostasis through the unfolded protein
    response (UPR). Upon accumulation of misfolded proteins in the ER, IRE1 forms
    clusters on the ER membrane to initiate UPR signaling. What regulates IRE1 cluster
    formation is not fully understood. Here, we show that the ER lumenal domain (LD)
    of human IRE1α forms biomolecular condensates in vitro. IRE1α LD condensates were
    stabilized both by binding to unfolded polypeptides as well as by tethering to
    model membranes, suggesting their role in assembling IRE1α into signaling-competent
    stable clusters. Molecular dynamics simulations indicated that weak multivalent
    interactions drive IRE1α LD clustering. Mutagenesis experiments identified disordered
    regions in IRE1α LD to control its clustering in vitro and in cells. Importantly,
    dysregulated clustering of IRE1α mutants led to defects in IRE1α signaling. Our
    results revealed that disordered regions in IRE1α LD control its clustering and
    suggest their role as a common strategy in regulating protein assembly on membranes.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Paulina
      foaf_name: Kettel, Paulina
      foaf_surname: Kettel
  - foaf_Person:
      foaf_givenName: Laura
      foaf_name: Marosits, Laura
      foaf_surname: Marosits
  - foaf_Person:
      foaf_givenName: Elena
      foaf_name: Spinetti, Elena
      foaf_surname: Spinetti
  - foaf_Person:
      foaf_givenName: Michael
      foaf_name: Rechberger, Michael
      foaf_surname: Rechberger
  - foaf_Person:
      foaf_givenName: Caterina
      foaf_name: Giannini, Caterina
      foaf_surname: Giannini
      foaf_workInfoHomepage: http://www.librecat.org/personId=e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
  - foaf_Person:
      foaf_givenName: Philipp
      foaf_name: Radler, Philipp
      foaf_surname: Radler
      foaf_workInfoHomepage: http://www.librecat.org/personId=40136C2A-F248-11E8-B48F-1D18A9856A87
    orcid: '0000-0001-9198-2182 '
  - foaf_Person:
      foaf_givenName: Isabell
      foaf_name: Niedermoser, Isabell
      foaf_surname: Niedermoser
  - foaf_Person:
      foaf_givenName: Irmgard
      foaf_name: Fischer, Irmgard
      foaf_surname: Fischer
  - foaf_Person:
      foaf_givenName: Gijs A.
      foaf_name: Versteeg, Gijs A.
      foaf_surname: Versteeg
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Loose, Martin
      foaf_surname: Loose
      foaf_workInfoHomepage: http://www.librecat.org/personId=462D4284-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7309-9724
  - foaf_Person:
      foaf_givenName: Roberto
      foaf_name: Covino, Roberto
      foaf_surname: Covino
  - foaf_Person:
      foaf_givenName: G. Elif
      foaf_name: Karagöz, G. Elif
      foaf_surname: Karagöz
  bibo_doi: 10.1038/s44318-024-00207-0
  bibo_issue: '20'
  bibo_volume: 43
  dct_date: 2024^xs_gYear
  dct_identifier:
  - UT:001306286100002
  dct_isPartOf:
  - http://id.crossref.org/issn/0261-4189
  - http://id.crossref.org/issn/1460-2075
  dct_language: eng
  dct_publisher: Embo Press@
  dct_title: Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced
    clustering@
  fabio_hasPubmedId: '39232130'
...