{"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"year":"2024","article_number":"116807","scopus_import":"1","abstract":[{"lang":"eng","text":"This study presents a graphene field-effect transistor (gFET) biosensor with dual detection capabilities for SARS-CoV-2: one RNA detection assay to confirm viral positivity and the other for nucleocapsid (N-)protein detection as a proxy for infectiousness of the patient. This technology can be rapidly adapted to emerging infectious diseases, making an essential tool to contain future pandemics. To detect viral RNA, the highly conserved E-gene of the virus was targeted, allowing for the determination of SARS-CoV-2 presence or absence using nasopharyngeal swab samples. For N-protein detection, specific antibodies were used. Tested on 213 clinical nasopharyngeal samples, the gFET biosensor showed good correlation with RT-PCR cycle threshold values, proving its high sensitivity in detecting SARS-CoV-2 RNA. Specificity was confirmed using 21 pre-pandemic samples positive for other respiratory viruses. The gFET biosensor had a limit of detection (LOD) for N-protein of 0.9 pM, establishing a foundation for the development of a sensitive tool for monitoring active viral infection. Results of gFET based N-protein detection corresponded to the results of virus culture in all 16 available clinical samples and thus it also proved its capability to serve as a proxy for infectivity. Overall, these findings support the potential of the gFET biosensor as a point-of-care device for rapid diagnosis of SARS-CoV-2 infection and indirect assessment of infectiousness in patients, providing additional information for clinical and public health decision-making."}],"external_id":{"pmid":["39341071"]},"pmid":1,"title":"Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements","article_processing_charge":"Yes (in subscription journal)","_id":"18170","date_updated":"2024-10-07T10:40:48Z","article_type":"original","month":"09","publication_status":"published","date_published":"2024-09-27T00:00:00Z","status":"public","citation":{"ista":"Herdina AN, Bozdogan A, Aspermair P, Dostalek J, Klausberger M, Lingg N, Cserjan-Puschmann M, Aguilar PP, Auer S, Demirtas H, Andersson J, Lötsch F, Holzer B, Steinrigl A, Thalhammer F, Schellnegger J, Breuer M, Knoll W, Strassl R. 2024. Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements. Biosensors and Bioelectronics. 267, 116807.","ieee":"A. N. Herdina <i>et al.</i>, “Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements,” <i>Biosensors and Bioelectronics</i>, vol. 267. Elsevier, 2024.","apa":"Herdina, A. N., Bozdogan, A., Aspermair, P., Dostalek, J., Klausberger, M., Lingg, N., … Strassl, R. (2024). Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements. <i>Biosensors and Bioelectronics</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bios.2024.116807\">https://doi.org/10.1016/j.bios.2024.116807</a>","mla":"Herdina, Anna Nele, et al. “Bridging Basic Science and Applied Diagnostics: Comprehensive Viral Diagnostics Enabled by Graphene-Based Electronic Biosensor Technology Advancements.” <i>Biosensors and Bioelectronics</i>, vol. 267, 116807, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.bios.2024.116807\">10.1016/j.bios.2024.116807</a>.","ama":"Herdina AN, Bozdogan A, Aspermair P, et al. Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements. <i>Biosensors and Bioelectronics</i>. 2024;267. doi:<a href=\"https://doi.org/10.1016/j.bios.2024.116807\">10.1016/j.bios.2024.116807</a>","short":"A.N. Herdina, A. Bozdogan, P. Aspermair, J. Dostalek, M. Klausberger, N. Lingg, M. Cserjan-Puschmann, P.P. Aguilar, S. Auer, H. Demirtas, J. Andersson, F. Lötsch, B. Holzer, A. Steinrigl, F. Thalhammer, J. Schellnegger, M. Breuer, W. Knoll, R. Strassl, Biosensors and Bioelectronics 267 (2024).","chicago":"Herdina, Anna Nele, Anil Bozdogan, Patrik Aspermair, Jakub Dostalek, Miriam Klausberger, Nico Lingg, Monika Cserjan-Puschmann, et al. “Bridging Basic Science and Applied Diagnostics: Comprehensive Viral Diagnostics Enabled by Graphene-Based Electronic Biosensor Technology Advancements.” <i>Biosensors and Bioelectronics</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.bios.2024.116807\">https://doi.org/10.1016/j.bios.2024.116807</a>."},"author":[{"full_name":"Herdina, Anna Nele","first_name":"Anna Nele","last_name":"Herdina"},{"full_name":"Bozdogan, Anil","last_name":"Bozdogan","first_name":"Anil"},{"full_name":"Aspermair, Patrik","first_name":"Patrik","last_name":"Aspermair"},{"last_name":"Dostalek","first_name":"Jakub","full_name":"Dostalek, Jakub"},{"first_name":"Miriam","last_name":"Klausberger","full_name":"Klausberger, Miriam"},{"first_name":"Nico","last_name":"Lingg","full_name":"Lingg, Nico"},{"full_name":"Cserjan-Puschmann, Monika","last_name":"Cserjan-Puschmann","first_name":"Monika"},{"full_name":"Aguilar, Patricia Pereira","last_name":"Aguilar","first_name":"Patricia Pereira"},{"last_name":"Auer","first_name":"Simone","full_name":"Auer, Simone"},{"last_name":"Demirtas","first_name":"Halil","full_name":"Demirtas, Halil"},{"full_name":"Andersson, Jakob","id":"3a5f4167-9bd9-11ed-bd12-a1446d38776f","first_name":"Jakob","last_name":"Andersson"},{"last_name":"Lötsch","first_name":"Felix","full_name":"Lötsch, Felix"},{"full_name":"Holzer, Barbara","first_name":"Barbara","last_name":"Holzer"},{"full_name":"Steinrigl, Adi","last_name":"Steinrigl","first_name":"Adi"},{"first_name":"Florian","last_name":"Thalhammer","full_name":"Thalhammer, Florian"},{"full_name":"Schellnegger, Julia","last_name":"Schellnegger","first_name":"Julia"},{"full_name":"Breuer, Monika","first_name":"Monika","last_name":"Breuer"},{"last_name":"Knoll","first_name":"Wolfgang","full_name":"Knoll, Wolfgang"},{"first_name":"Robert","last_name":"Strassl","full_name":"Strassl, Robert"}],"language":[{"iso":"eng"}],"quality_controlled":"1","has_accepted_license":"1","publication_identifier":{"eissn":["1873-4235"],"issn":["0956-5663"]},"volume":267,"oa":1,"type":"journal_article","acknowledgement":"This research was funded in whole by the Austrian Science Fund (FWF) [P 35103-B, Grant-DOI: 10.55776/P35103]. For open access purposes, the author has applied a CC BY public copyright license to any author-accepted manuscript version arising from this submission. We would like to thank Olfert Landt for advice on ssDNA probe design; Rui Qiang Chen, Jennifer Stock, and Christine Wukotitsch for their excellent support with ONT sequencing; Christoph Köppl and Andreas Fischer for excellent support in recombinant N protein expression and purification; and the whole team at the division of clinical virology for their support with standard diagnostics.","intvolume":"       267","date_created":"2024-10-06T22:01:11Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"LeSa"}],"doi":"10.1016/j.bios.2024.116807","oa_version":"Published Version","publication":"Biosensors and Bioelectronics","publisher":"Elsevier","day":"27","main_file_link":[{"url":"https://doi.org/10.1016/j.bios.2024.116807","open_access":"1"}]}