---
res:
  bibo_abstract:
  - It is widely believed that information storage in neuronal circuits involves nanoscopic
    structural changes at synapses, resulting in the formation of synaptic engrams.
    However, direct evidence for this hypothesis is lacking. To test this conjecture,
    we combined chemical potentiation, functional analysis by paired pre-postsynaptic
    recordings, and structural analysis by electron microscopy (EM) and freeze-fracture
    replica labeling (FRL) at the murine hippocampal mossy fiber synapse, a key synapse
    in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic
    transmission revealed that forskolin-induced chemical potentiation increased the
    readily releasable vesicle pool size and vesicular release probability by 146%
    and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL
    demonstrated an increase in the number of vesicles close to the plasma membrane
    and the number of clusters of the priming protein Munc13-1, indicating an increase
    in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed
    a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels,
    suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our
    results indicate that presynaptic plasticity is associated with structural reorganization
    of active zones. We propose that changes in potential nanoscopic organization
    at synaptic vesicle release sites may be correlates of learning and memory at
    a plastic central synapse.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Olena
      foaf_name: Kim, Olena
      foaf_surname: Kim
      foaf_workInfoHomepage: http://www.librecat.org/personId=3F8ABDDA-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-2344-1039
  bibo_doi: 10.15479/AT:ISTA:18296
  dct_date: 2024^xs_gYear
  dct_publisher: Institute of Science and Technology Austria@
  dct_subject:
  - Hippocampal mossy fiber synapses
  - short-term potentiation
  - long-term potentiation
  - presynaptic plasticity
  - electron microscopy
  - freeze-fracture replica labeling
  - paired recordings
  - forskolin
  - cyclic adenosine monophosphate (cAMP)
  - protein kinase A (PKA)
  - neuromodulation
  - synaptic vesicle pools
  - presynaptic Ca2+ channels
  - Munc13
  - docking
  - priming
  - active zone
  dct_title: Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of
    synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber
    boutons@
...