---
res:
  bibo_abstract:
  - Multivesicular endosomes (MVEs) sequester membrane proteins destined for degradation
    within intralumenal vesicles (ILVs), a process mediated by the membrane-remodeling
    action of Endosomal Sorting Complex Required for Transport (ESCRT) proteins. In
    Arabidopsis, endosomal membrane constriction and scission are uncoupled, resulting
    in the formation of extensive concatenated ILV networks and enhancing cargo sequestration
    efficiency. Here, we used a combination of electron tomography, computer simulations,
    and mathematical modeling to address the questions of when concatenated ILV networks
    evolved in plants and what drives their formation. Through morphometric analyses
    of tomographic reconstructions of endosomes across yeast, algae, and various land
    plants, we have found that ILV concatenation is widespread within plant species,
    but only prevalent in seed plants, especially in flowering plants. Multiple budding
    sites that require the formation of pores in the limiting membrane were only identified
    in hornworts and seed plants, suggesting that this mechanism has evolved independently
    in both plant lineages. To identify the conditions under which these multiple
    budding sites can arise, we used particle-based molecular dynamics simulations
    and found that changes in ESCRT filament properties, such as filament curvature
    and membrane binding energy, can generate the membrane shapes observed in multiple
    budding sites. To understand the relationship between membrane budding activity
    and ILV network topology, we performed computational simulations and identified
    a set of membrane remodeling parameters that can recapitulate our tomographic
    datasets.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Ethan
      foaf_name: Weiner, Ethan
      foaf_surname: Weiner
  - foaf_Person:
      foaf_givenName: Elizabeth
      foaf_name: Berryman, Elizabeth
      foaf_surname: Berryman
  - foaf_Person:
      foaf_givenName: Felix F
      foaf_name: Frey, Felix F
      foaf_surname: Frey
      foaf_workInfoHomepage: http://www.librecat.org/personId=a0270b37-8f1a-11ec-95c7-8e710c59a4f3
    orcid: 0000-0001-8501-6017
  - foaf_Person:
      foaf_givenName: Ariadna González
      foaf_name: Solís, Ariadna González
      foaf_surname: Solís
  - foaf_Person:
      foaf_givenName: André
      foaf_name: Leier, André
      foaf_surname: Leier
  - foaf_Person:
      foaf_givenName: Tatiana Marquez
      foaf_name: Lago, Tatiana Marquez
      foaf_surname: Lago
  - foaf_Person:
      foaf_givenName: Anđela
      foaf_name: Šarić, Anđela
      foaf_surname: Šarić
      foaf_workInfoHomepage: http://www.librecat.org/personId=bf63d406-f056-11eb-b41d-f263a6566d8b
    orcid: 0000-0002-7854-2139
  - foaf_Person:
      foaf_givenName: Marisa S.
      foaf_name: Otegui, Marisa S.
      foaf_surname: Otegui
  bibo_doi: 10.1073/pnas.2409407121
  bibo_issue: '44'
  bibo_volume: 121
  dct_date: 2024^xs_gYear
  dct_identifier:
  - UT:001349500800007
  dct_isPartOf:
  - http://id.crossref.org/issn/0027-8424
  - http://id.crossref.org/issn/1091-6490
  dct_language: eng
  dct_publisher: National Academy of Sciences@
  dct_title: Endosomal membrane budding patterns in plants@
  fabio_hasPubmedId: '39441629'
...