{"publisher":"Elsevier","title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis","date_published":"2024-12-02T00:00:00Z","pmid":1,"publication":"Molecular Plant","language":[{"iso":"eng"}],"publication_status":"published","day":"02","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"file_date_updated":"2024-12-03T11:08:09Z","status":"public","abstract":[{"lang":"eng","text":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis."}],"quality_controlled":"1","intvolume":"        17","oa":1,"ddc":["580"],"doi":"10.1016/j.molp.2024.11.001","acknowledgement":"We dedicate this paper to the deceased Petr Galuszka for his inspiration and support of our project. We thank Prof. Peter Hedden for constructive criticism of the manuscript and English editing. No conflict of interest is declared.","month":"12","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"OA_type":"hybrid","volume":17,"year":"2024","external_id":{"pmid":["39501563"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"publisher","oa_version":"Published Version","file":[{"date_created":"2024-12-03T11:08:09Z","date_updated":"2024-12-03T11:08:09Z","checksum":"a11feea4b1677df76b632eca04bfc1dd","file_id":"18612","access_level":"open_access","file_size":3308945,"creator":"dernst","success":1,"content_type":"application/pdf","relation":"main_file","file_name":"2024_MolecularPlant_Kralova.pdf"}],"_id":"18596","issue":"12","article_processing_charge":"Yes (in subscription journal)","citation":{"apa":"Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., … Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>","chicago":"Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>.","mla":"Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>, vol. 17, no. 12, Elsevier, 2024, pp. 1850–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>.","ieee":"M. Králová <i>et al.</i>, “A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis,” <i>Molecular Plant</i>, vol. 17, no. 12. Elsevier, pp. 1850–1865, 2024.","ama":"Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. 2024;17(12):1850-1865. doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>","ista":"Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.","short":"M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C. Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml, O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865."},"article_type":"original","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","scopus_import":"1","date_updated":"2024-12-03T11:09:39Z","date_created":"2024-11-28T11:13:35Z","type":"journal_article","has_accepted_license":"1","publication_identifier":{"issn":["1674-2052"]},"author":[{"first_name":"Michaela","full_name":"Králová, Michaela","last_name":"Králová"},{"last_name":"Kubalová","first_name":"Ivona","full_name":"Kubalová, Ivona"},{"full_name":"Hajný, Jakub","first_name":"Jakub","last_name":"Hajný"},{"full_name":"Kubiasova, Karolina","orcid":"0000-0001-5630-9419","first_name":"Karolina","id":"946011F4-3E71-11EA-860B-C7A73DDC885E","last_name":"Kubiasova"},{"first_name":"Karolína","full_name":"Vagaská, Karolína","last_name":"Vagaská"},{"id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge","full_name":"Ge, Zengxiang","first_name":"Zengxiang","orcid":"0000-0001-9381-3577"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368"},{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","last_name":"Semerádová","full_name":"Semerádová, Hana","first_name":"Hana"},{"last_name":"Kuchařová","full_name":"Kuchařová, Anna","first_name":"Anna"},{"first_name":"Martin","full_name":"Hönig, Martin","last_name":"Hönig"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline","full_name":"Monzer, Aline"},{"full_name":"Kovačik, Martin","first_name":"Martin","last_name":"Kovačik"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Novák","first_name":"Ondřej","full_name":"Novák, Ondřej"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"last_name":"Ikeda","full_name":"Ikeda, Yoshihisa","first_name":"Yoshihisa"},{"first_name":"David","full_name":"Zalabák, David","last_name":"Zalabák"}],"page":"1850-1865"}