---
OA_place: publisher
OA_type: hybrid
_id: '18596'
abstract:
- lang: eng
text: Hormone perception and signaling pathways have a fundamental regulatory function
in the physiological processes of plants. Cytokinins, a class of plant hormones,
regulate cell division and meristem maintenance. The cytokinin signaling pathway
is well established in the model plant Arabidopsis thaliana. Several negative
feedback mechanisms, tightly controlling cytokinin signaling output, have been
described previously. In this study, we identified a new feedback mechanism executed
through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing
variant named CRE1int7 results from seventh intron retention, introducing a premature
termination codon in the transcript. We showed that CRE1int7 is translated in
planta into a truncated receptor lacking the C-terminal receiver domain essential
for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream
cascade. We present a novel negative feedback mechanism of the cytokinin signaling
pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin
receptors via dimerization and compete with them for ligand binding. Ensuring
proper plant growth and development requires precise control of the cytokinin
signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism
for fine-tuning the cytokinin signaling pathway in Arabidopsis.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We dedicate this paper to the deceased Petr Galuszka for his inspiration
and support of our project. We thank Prof. Peter Hedden for constructive criticism
of the manuscript and English editing. No conflict of interest is declared.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Michaela
full_name: Králová, Michaela
last_name: Králová
- first_name: Ivona
full_name: Kubalová, Ivona
last_name: Kubalová
- first_name: Jakub
full_name: Hajný, Jakub
last_name: Hajný
- first_name: Karolina
full_name: Kubiasova, Karolina
id: 946011F4-3E71-11EA-860B-C7A73DDC885E
last_name: Kubiasova
orcid: 0000-0001-5630-9419
- first_name: Karolína
full_name: Vagaská, Karolína
last_name: Vagaská
- first_name: Zengxiang
full_name: Ge, Zengxiang
id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8
last_name: Ge
orcid: 0000-0001-9381-3577
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Hana
full_name: Semerádová, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semerádová
- first_name: Anna
full_name: Kuchařová, Anna
last_name: Kuchařová
- first_name: Martin
full_name: Hönig, Martin
last_name: Hönig
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Martin
full_name: Kovačik, Martin
last_name: Kovačik
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Yoshihisa
full_name: Ikeda, Yoshihisa
last_name: Ikeda
- first_name: David
full_name: Zalabák, David
last_name: Zalabák
citation:
ama: Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative
splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant.
2024;17(12):1850-1865. doi:10.1016/j.molp.2024.11.001
apa: Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., …
Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2024.11.001
chicago: Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína
Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from
Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular
Plant. Elsevier, 2024. https://doi.org/10.1016/j.molp.2024.11.001.
ieee: M. Králová et al., “A decoy receptor derived from alternative splicing
fine-tunes cytokinin signaling in Arabidopsis,” Molecular Plant, vol. 17,
no. 12. Elsevier, pp. 1850–1865, 2024.
ista: Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová
H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda
Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.
mla: Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing
Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular Plant, vol. 17,
no. 12, Elsevier, 2024, pp. 1850–65, doi:10.1016/j.molp.2024.11.001.
short: M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C.
Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml,
O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.
date_created: 2024-11-28T11:13:35Z
date_published: 2024-12-02T00:00:00Z
date_updated: 2025-09-08T14:46:45Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.molp.2024.11.001
external_id:
isi:
- '001373778300001'
pmid:
- '39501563'
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intvolume: ' 17'
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issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 1850-1865
pmid: 1
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling
in Arabidopsis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
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(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
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volume: 17
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...