---
APC_amount: 804 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18601'
abstract:
- lang: eng
  text: "Geometrically controlled stem cell differentiation promotes reproducible
    pattern formation. Here, we present a protocol to fabricate elastomeric stencils
    for patterned stem cell differentiation. We describe procedures for using photolithography
    to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils
    with through holes. We then provide instructions for culturing cells on stencils
    and, finally, removing stencils to allow colony growth and cell migration. This
    approach yields reproducible two-dimensional organoids tailored for quantitative
    studies of growth and pattern formation.\r\nFor complete details on the use and
    execution of this protocol, please refer to Lehr et al.1"
acknowledged_ssus:
- _id: NanoFab
acknowledgement: We thank the nanofabrication facility at ISTA for technical assistance.
  Work in the A.K. lab is supported by ISTA, the European Research Council under Horizon
  Europe (grant 101044579), and the Austrian Science Fund (FWF) (grant https://doi.org/10.55776/F78).
  S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H.
  fellowship SC19-011.
article_number: '103187'
article_processing_charge: Yes
article_type: original
author:
- first_name: Stefanie
  full_name: Rus, Stefanie
  id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
  last_name: Rus
  orcid: 0000-0001-8703-1093
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Monika Aleksandra
  full_name: Kulig, Monika Aleksandra
  id: 3331f5ae-e896-11ec-af79-eeb79769bcb7
  last_name: Kulig
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. Protocol for fabricating
    elastomeric stencils for patterned stem cell differentiation. <i>STAR Protocols</i>.
    2024;5(4). doi:<a href="https://doi.org/10.1016/j.xpro.2024.103187">10.1016/j.xpro.2024.103187</a>
  apa: Rus, S., Merrin, J., Kulig, M. A., Minchington, T., &#38; Kicheva, A. (2024).
    Protocol for fabricating elastomeric stencils for patterned stem cell differentiation.
    <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2024.103187">https://doi.org/10.1016/j.xpro.2024.103187</a>
  chicago: Rus, Stefanie, Jack Merrin, Monika Aleksandra Kulig, Thomas Minchington,
    and Anna Kicheva. “Protocol for Fabricating Elastomeric Stencils for Patterned
    Stem Cell Differentiation.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2024.103187">https://doi.org/10.1016/j.xpro.2024.103187</a>.
  ieee: S. Rus, J. Merrin, M. A. Kulig, T. Minchington, and A. Kicheva, “Protocol
    for fabricating elastomeric stencils for patterned stem cell differentiation,”
    <i>STAR Protocols</i>, vol. 5, no. 4. Elsevier, 2024.
  ista: Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. 2024. Protocol for fabricating
    elastomeric stencils for patterned stem cell differentiation. STAR Protocols.
    5(4), 103187.
  mla: Rus, Stefanie, et al. “Protocol for Fabricating Elastomeric Stencils for Patterned
    Stem Cell Differentiation.” <i>STAR Protocols</i>, vol. 5, no. 4, 103187, Elsevier,
    2024, doi:<a href="https://doi.org/10.1016/j.xpro.2024.103187">10.1016/j.xpro.2024.103187</a>.
  short: S. Rus, J. Merrin, M.A. Kulig, T. Minchington, A. Kicheva, STAR Protocols
    5 (2024).
corr_author: '1'
date_created: 2024-12-01T23:01:53Z
date_published: 2024-12-20T00:00:00Z
date_updated: 2026-08-06T22:30:36Z
day: '20'
ddc:
- '570'
department:
- _id: AnKi
- _id: NanoFab
doi: 10.1016/j.xpro.2024.103187
external_id:
  pmid:
  - '39602310'
file:
- access_level: open_access
  checksum: 0c61a6f9978608a103865905e06f4581
  content_type: application/pdf
  creator: dernst
  date_created: 2024-12-03T10:53:23Z
  date_updated: 2024-12-03T10:53:23Z
  file_id: '18610'
  file_name: 2024_STARProtoc_Lehr.pdf
  file_size: 4989169
  relation: main_file
  success: 1
file_date_updated: 2024-12-03T10:53:23Z
has_accepted_license: '1'
intvolume: '         5'
issue: '4'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
  grant_number: '101044579'
  name: Mechanisms of tissue size regulation in spinal cord development
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
  grant_number: SC19-011
  name: The regulatory logic of pattern formation in the vertebrate dorsal neural
    tube
publication: STAR Protocols
publication_identifier:
  eissn:
  - 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '19763'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Protocol for fabricating elastomeric stencils for patterned stem cell differentiation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...