{"article_type":"original","oa_version":"Published Version","doi":"10.1242/dev.201208","publisher":"The Company of Biologists","pmid":1,"date_published":"2023-09-18T00:00:00Z","oa":1,"scopus_import":"1","file_date_updated":"2024-12-04T22:12:04Z","publication":"Development","file":[{"content_type":"application/pdf","file_name":"dev201208.pdf","date_created":"2024-12-04T22:12:04Z","access_level":"open_access","creator":"nramirez","success":1,"checksum":"d2158dc56db50457e6404c4afec4401c","date_updated":"2024-12-04T22:12:04Z","file_size":9559527,"relation":"main_file","file_id":"18624"}],"date_created":"2024-12-04T22:02:52Z","status":"public","extern":"1","_id":"18621","issue":"18","title":"Convertase-dependent regulation of membrane-tethered and secreted ligands tunes dendrite adhesion","publication_status":"published","intvolume":"       150","acknowledgement":"We thank members of the Bülow laboratory for comments on the manuscript and discussions throughout the course of this work; and Ryan Peer and William Corman for their initial help with the modifier genetic screen. We acknowledge the Genomics Core facility and the Advanced Imaging Facility at Albert Einstein College of Medicine for help during these studies. We are grateful to Kang Shen, David Miller and the Caenorhabditis Genetics Center (which is funded by National Institutes of Health Office of Research Infrastructure Programs P40OD0104400) for some of the strains used in this study, and Lhisia Chen for the anti-SAX-7 antibody.\r\nThis work was supported by grants from the National Institutes of Health (F31NS100370 to M.R.; T32GM007288 and F31NS111939 to M.T.; R01NS096672, R21NS081505 and R01NS129992 to H.E.B.; and P30HD071593 to Albert Einstein College of Medicine). N.J.R.-S. was the recipient of a Colciencias-Fulbright Fellowship [funded by Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) and Fulbright Colombia], L.T.H.T. of a Croucher Foundation Fellowship, and H.E.B. of an Irma T. Hirschl Trust/Monique Weill-Caulier Trust research fellowship. Open Access funding provided by Albert Einstein College of Medicine, Yeshiva University. Deposited in PMC for immediate release.","year":"2023","volume":150,"month":"09","type":"journal_article","OA_place":"publisher","ddc":["570"],"OA_type":"hybrid","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"date_updated":"2024-12-09T11:43:40Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["37721334"]},"abstract":[{"text":"During neural development, cellular adhesion is crucial for interactions among and between neurons and surrounding tissues. This function is mediated by conserved cell adhesion molecules, which are tightly regulated to allow for coordinated neuronal outgrowth. Here, we show that the proprotein convertase KPC-1 (homolog of mammalian furin) regulates the Menorin adhesion complex during development of PVD dendritic arbors in Caenorhabditis elegans. We found a finely regulated antagonistic balance between PVD-expressed KPC-1 and the epidermally expressed putative cell adhesion molecule MNR-1 (Menorin). Genetically, partial loss of mnr-1 suppressed partial loss of kpc-1, and both loss of kpc-1 and transgenic overexpression of mnr-1 resulted in indistinguishable phenotypes in PVD dendrites. This balance regulated cell-surface localization of the DMA-1 leucine-rich transmembrane receptor in PVD neurons. Lastly, kpc-1 mutants showed increased amounts of MNR-1 and decreased amounts of muscle-derived LECT-2 (Chondromodulin II), which is also part of the Menorin adhesion complex. These observations suggest that KPC-1 in PVD neurons directly or indirectly controls the abundance of proteins of the Menorin adhesion complex from adjacent tissues, thereby providing negative feedback from the dendrite to the instructive cues of surrounding tissues.","lang":"eng"}],"language":[{"iso":"eng"}],"article_processing_charge":"No","has_accepted_license":"1","quality_controlled":"1","author":[{"first_name":"Nelson","last_name":"Ramirez","id":"39831956-E4FE-11E9-85DE-0DC7E5697425","full_name":"Ramirez, Nelson"},{"full_name":"Belalcazar, Helen M.","last_name":"Belalcazar","first_name":"Helen M."},{"full_name":"Rahman, Maisha","last_name":"Rahman","first_name":"Maisha"},{"full_name":"Trivedi, Meera","first_name":"Meera","last_name":"Trivedi"},{"full_name":"Tang, Leo T. H.","last_name":"Tang","first_name":"Leo T. H."},{"full_name":"Bülow, Hannes E.","last_name":"Bülow","first_name":"Hannes E."}],"citation":{"short":"N. Ramirez, H.M. Belalcazar, M. Rahman, M. Trivedi, L.T.H. Tang, H.E. Bülow, Development 150 (2023).","ama":"Ramirez N, Belalcazar HM, Rahman M, Trivedi M, Tang LTH, Bülow HE. Convertase-dependent regulation of membrane-tethered and secreted ligands tunes dendrite adhesion. <i>Development</i>. 2023;150(18). doi:<a href=\"https://doi.org/10.1242/dev.201208\">10.1242/dev.201208</a>","apa":"Ramirez, N., Belalcazar, H. M., Rahman, M., Trivedi, M., Tang, L. T. H., &#38; Bülow, H. E. (2023). Convertase-dependent regulation of membrane-tethered and secreted ligands tunes dendrite adhesion. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.201208\">https://doi.org/10.1242/dev.201208</a>","ieee":"N. Ramirez, H. M. Belalcazar, M. Rahman, M. Trivedi, L. T. H. Tang, and H. E. Bülow, “Convertase-dependent regulation of membrane-tethered and secreted ligands tunes dendrite adhesion,” <i>Development</i>, vol. 150, no. 18. The Company of Biologists, 2023.","ista":"Ramirez N, Belalcazar HM, Rahman M, Trivedi M, Tang LTH, Bülow HE. 2023. Convertase-dependent regulation of membrane-tethered and secreted ligands tunes dendrite adhesion. Development. 150(18).","chicago":"Ramirez, Nelson, Helen M. Belalcazar, Maisha Rahman, Meera Trivedi, Leo T. H. Tang, and Hannes E. Bülow. “Convertase-Dependent Regulation of Membrane-Tethered and Secreted Ligands Tunes Dendrite Adhesion.” <i>Development</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/dev.201208\">https://doi.org/10.1242/dev.201208</a>.","mla":"Ramirez, Nelson, et al. “Convertase-Dependent Regulation of Membrane-Tethered and Secreted Ligands Tunes Dendrite Adhesion.” <i>Development</i>, vol. 150, no. 18, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/dev.201208\">10.1242/dev.201208</a>."},"day":"18"}