{"language":[{"iso":"eng"}],"tmp":{"image":"/images/cc_by_sa.png","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_published":"2024-12-17T00:00:00Z","status":"public","date_created":"2024-12-16T10:53:39Z","date_updated":"2025-07-04T08:09:55Z","month":"12","author":[{"id":"4f2d02dd-47a9-11ec-ad10-82820ed3f501","full_name":"Santana de Freitas Amaral, Miguel","first_name":"Miguel","last_name":"Santana de Freitas Amaral"}],"related_material":{"record":[{"relation":"part_of_dissertation","id":"18670","status":"public"}]},"OA_place":"repository","oa":1,"publication_identifier":{"isbn":["978-3-99078-046-6"],"issn":["2663-337X"]},"publisher":"Institute of Science and Technology Austria","has_accepted_license":"1","file_date_updated":"2025-06-18T22:30:03Z","type":"dissertation","supervisor":[{"first_name":"Anđela","last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","orcid":"0000-0002-7854-2139"}],"year":"2024","title":"Archaeal membranes : In silico modelling and design","department":[{"_id":"GradSch"},{"_id":"AnSa"}],"day":"17","publication_status":"published","corr_author":"1","license":"https://creativecommons.org/licenses/by-sa/4.0/","ddc":["572","530"],"oa_version":"Published Version","doi":"10.15479/at:ista:18661","abstract":[{"text":"Across the tree of life, distinct designs of cellular membranes have evolved that are both stable\r\nand flexible. In bacteria and eukaryotes this trade-off is accomplished by single-headed lipids\r\nthat self-assemble into flexible bilayer membranes. By contrast, archaea in many cases possess\r\nboth bilayer and double-headed, monolayer spanning bolalipids. This composition is believed\r\nto enable extremophile archaea to survive harsh environments. Here, through the creation of a\r\nminimal computational model for bolalipid membranes, we discover trade-offs when forming\r\nmembranes using lipids of a single type. Similar to living archaea, we can tune the stiffness of\r\nbolalipid molecules. We find that membranes made out of flexible bolalipid molecules resemble\r\nbilayer membranes as they can adopt U-shaped conformations to enable higher curvatures.\r\nConversely, rigid bolalipid molecules, like those found in archaea at higher temperatures,\r\npreferentially take on a straight conformation to self-assemble into liquid membranes that are\r\nstable, stiff, prone to pore formation, and which tear during membrane reshaping. Strikingly,\r\nhowever, our analysis reveals that it is possible to achieve the best of both worlds – membranes\r\nthat are fluid, stable at high temperatures and flexible enough to be reshaped without leaking –\r\nthrough the inclusion of a small fraction of bilayer lipids into a bolalipid membrane. Additionally,\r\nthe curvature-dependent softening of bolalipid membranes made of lipids with tension-sensitive\r\nconformation can also enable high rigidity at low curvatures while softening at high curvatures,\r\nmaking the membrane effectively a plastic material. Taken together, our study compares the\r\ndifferent membrane designs across the tree of life and indicates how combining lipids can be\r\nused to resolve trade-offs when generating membranes for (bio)technological applications.\r\n","lang":"eng"}],"_id":"18661","citation":{"apa":"Santana de Freitas Amaral, M. (2024). <i>Archaeal membranes : In silico modelling and design</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:18661\">https://doi.org/10.15479/at:ista:18661</a>","ieee":"M. Santana de Freitas Amaral, “Archaeal membranes : In silico modelling and design,” Institute of Science and Technology Austria, 2024.","ama":"Santana de Freitas Amaral M. Archaeal membranes : In silico modelling and design. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:18661\">10.15479/at:ista:18661</a>","mla":"Santana de Freitas Amaral, Miguel. <i>Archaeal Membranes : In Silico Modelling and Design</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:18661\">10.15479/at:ista:18661</a>.","chicago":"Santana de Freitas Amaral, Miguel. “Archaeal Membranes : In Silico Modelling and Design.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:18661\">https://doi.org/10.15479/at:ista:18661</a>.","ista":"Santana de Freitas Amaral M. 2024. Archaeal membranes : In silico modelling and design. Institute of Science and Technology Austria.","short":"M. Santana de Freitas Amaral, Archaeal Membranes : In Silico Modelling and Design, Institute of Science and Technology Austria, 2024."},"file":[{"date_created":"2024-12-18T12:27:01Z","creator":"mamaral","file_id":"18671","checksum":"eca06497a29078558395455c890a32d9","embargo_to":"open_access","file_size":19161387,"date_updated":"2025-06-18T22:30:03Z","relation":"source_file","access_level":"closed","content_type":"application/zip","file_name":"2024_msfa_thesis.zip"},{"content_type":"application/pdf","embargo":"2025-06-18","file_name":"2024_msfa_thesis.pdf","relation":"main_file","access_level":"open_access","checksum":"2dc30ea46c5daf48d07e4cccb3c3de00","date_updated":"2025-06-18T22:30:03Z","file_size":16530084,"date_created":"2024-12-18T12:26:30Z","creator":"mamaral","file_id":"18672"}],"degree_awarded":"PhD","page":"57","article_processing_charge":"No","alternative_title":["ISTA Thesis"]}