{"intvolume":"       110","date_published":"2024-12-10T00:00:00Z","publication_identifier":{"issn":["2470-0045"],"eissn":["2470-0053"]},"date_updated":"2025-01-02T10:50:16Z","OA_place":"repository","arxiv":1,"title":"Coat stiffening can explain invagination of clathrin-coated membranes","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the main pathway used by eukaryotic cells to take up extracellular material, but the dominant physical mechanisms driving this process are still elusive. Recently, several high-resolution imaging techniques have been used on different cell lines to measure the geometrical properties of clathrin-coated pits over their whole lifetime. Here, we first show that the combination of all datasets with the recently introduced cooperative curvature model defines a consensus pathway, which is characterized by a flat-to-curved transition at finite area, followed by linear growth and subsequent saturation of curvature. We then apply an energetic model for the composite of the plasma membrane and clathrin coat to this consensus pathway to show that the dominant mechanism for invagination could be coat stiffening, which might originate from cooperative interactions between the different clathrin molecules and progressively drives the system toward its intrinsic curvature. Our theory predicts that two length scales determine the invagination pathway, namely the patch size at which the flat-to-curved transition occurs and the final pit radius."}],"status":"public","type":"journal_article","publication_status":"published","publication":"Physical Review E","oa":1,"language":[{"iso":"eng"}],"OA_type":"green","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","doi":"10.1103/PhysRevE.110.064403","acknowledgement":"We thank Markus Mund, Aline Tschanz, and Jonas Ries for helpful discussions and a critical reading of the manuscript. We also kindly acknowledge Simon Scheuring for providing the HS-AFM data for the analysis of clathrin coat invagination. We thank the reviewers of previous versions of this manuscript for useful feedback that helped us to improve this work. F.F. acknowledges financial support by the NOMIS foundation. U.S.S. was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Project No. 240245660 (SFB 1129). Moreover, he is a member of the Interdisciplinary Center for Scientific Computing (IWR) at Heidelberg and of the Max Planck School Matter to Life supported by the German Federal Ministry of Education and Research (BMBF) in collaboration with the Max Planck Society.","_id":"18704","date_created":"2024-12-22T23:01:48Z","citation":{"mla":"Frey, Felix F., and Ulrich S. Schwarz. “Coat Stiffening Can Explain Invagination of Clathrin-Coated Membranes.” <i>Physical Review E</i>, vol. 110, no. 6, 064403, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/PhysRevE.110.064403\">10.1103/PhysRevE.110.064403</a>.","chicago":"Frey, Felix F, and Ulrich S. Schwarz. “Coat Stiffening Can Explain Invagination of Clathrin-Coated Membranes.” <i>Physical Review E</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/PhysRevE.110.064403\">https://doi.org/10.1103/PhysRevE.110.064403</a>.","ieee":"F. F. Frey and U. S. Schwarz, “Coat stiffening can explain invagination of clathrin-coated membranes,” <i>Physical Review E</i>, vol. 110, no. 6. American Physical Society, 2024.","short":"F.F. Frey, U.S. Schwarz, Physical Review E 110 (2024).","apa":"Frey, F. F., &#38; Schwarz, U. S. (2024). Coat stiffening can explain invagination of clathrin-coated membranes. <i>Physical Review E</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevE.110.064403\">https://doi.org/10.1103/PhysRevE.110.064403</a>","ista":"Frey FF, Schwarz US. 2024. Coat stiffening can explain invagination of clathrin-coated membranes. Physical Review E. 110(6), 064403.","ama":"Frey FF, Schwarz US. Coat stiffening can explain invagination of clathrin-coated membranes. <i>Physical Review E</i>. 2024;110(6). doi:<a href=\"https://doi.org/10.1103/PhysRevE.110.064403\">10.1103/PhysRevE.110.064403</a>"},"year":"2024","publisher":"American Physical Society","author":[{"first_name":"Felix F","orcid":"0000-0001-8501-6017","last_name":"Frey","id":"a0270b37-8f1a-11ec-95c7-8e710c59a4f3","full_name":"Frey, Felix F"},{"first_name":"Ulrich S.","full_name":"Schwarz, Ulrich S.","last_name":"Schwarz"}],"oa_version":"Preprint","department":[{"_id":"AnSa"}],"day":"10","scopus_import":"1","issue":"6","month":"12","article_type":"original","article_number":"064403","article_processing_charge":"No","volume":110,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.48550/arXiv.2405.02820"}],"external_id":{"arxiv":["2405.02820"]}}