---
res:
  bibo_abstract:
  - We provide theoretical tests of a novel experimental technique to determine mechanostability
    of proteins based on stretching a mechanically protected protein by single-molecule
    force spectroscopy. This technique involves stretching a homogeneous or heterogeneous
    chain of reference proteins (single-molecule markers) in which one of them acts
    as host to the guest protein under study. The guest protein is grafted into the
    host through genetic engineering. It is expected that unraveling of the host precedes
    the unraveling of the guest removing ambiguities in the reading of the force-extension
    patterns of the guest protein. We study examples of such systems within a coarse-grained
    structure-based model. We consider systems with various ratios of mechanostability
    for the host and guest molecules and compare them to experimental results involving
    cohesin I as the guest molecule. For a comparison, we also study the force-displacement
    patterns in proteins that are linked in a serial fashion. We find that the mechanostability
    of the guest is similar to that of the isolated or serially linked protein. We
    also demonstrate that the ideal configuration of this strategy would be one in
    which the host is much more mechanostable than the single-molecule markers. We
    finally show that it is troublesome to use the highly stable cystine knot proteins
    as a host to graft a guest in stretching studies because this would involve a
    cleaving procedure.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Mateusz
      foaf_name: Chwastyk, Mateusz
      foaf_surname: Chwastyk
  - foaf_Person:
      foaf_givenName: Albert
      foaf_name: Galera Prat, Albert
      foaf_surname: Galera Prat
  - foaf_Person:
      foaf_givenName: Mateusz K
      foaf_name: Sikora, Mateusz K
      foaf_surname: Sikora
      foaf_workInfoHomepage: http://www.librecat.org/personId=2F74BCDE-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Àngel
      foaf_name: Gómez Sicilia, Àngel
      foaf_surname: Gómez Sicilia
  - foaf_Person:
      foaf_givenName: Mariano
      foaf_name: Carrión Vázquez, Mariano
      foaf_surname: Carrión Vázquez
  - foaf_Person:
      foaf_givenName: Marek
      foaf_name: Cieplak, Marek
      foaf_surname: Cieplak
  bibo_doi: 10.1002/prot.24436
  bibo_issue: '5'
  bibo_volume: 82
  dct_date: 2014^xs_gYear
  dct_identifier:
  - UT:000337280400003
  dct_language: eng
  dct_publisher: Wiley-Blackwell@
  dct_title: Theoretical tests of the mechanical protection strategy in protein nanomechanics@
...