---
_id: '1891'
abstract:
- lang: eng
  text: We provide theoretical tests of a novel experimental technique to determine
    mechanostability of proteins based on stretching a mechanically protected protein
    by single-molecule force spectroscopy. This technique involves stretching a homogeneous
    or heterogeneous chain of reference proteins (single-molecule markers) in which
    one of them acts as host to the guest protein under study. The guest protein is
    grafted into the host through genetic engineering. It is expected that unraveling
    of the host precedes the unraveling of the guest removing ambiguities in the reading
    of the force-extension patterns of the guest protein. We study examples of such
    systems within a coarse-grained structure-based model. We consider systems with
    various ratios of mechanostability for the host and guest molecules and compare
    them to experimental results involving cohesin I as the guest molecule. For a
    comparison, we also study the force-displacement patterns in proteins that are
    linked in a serial fashion. We find that the mechanostability of the guest is
    similar to that of the isolated or serially linked protein. We also demonstrate
    that the ideal configuration of this strategy would be one in which the host is
    much more mechanostable than the single-molecule markers. We finally show that
    it is troublesome to use the highly stable cystine knot proteins as a host to
    graft a guest in stretching studies because this would involve a cleaving procedure.
acknowledgement: Grant Nr. 2011/01/N/ST3/02475
article_processing_charge: No
author:
- first_name: Mateusz
  full_name: Chwastyk, Mateusz
  last_name: Chwastyk
- first_name: Albert
  full_name: Galera Prat, Albert
  last_name: Galera Prat
- first_name: Mateusz K
  full_name: Sikora, Mateusz K
  id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
  last_name: Sikora
- first_name: Àngel
  full_name: Gómez Sicilia, Àngel
  last_name: Gómez Sicilia
- first_name: Mariano
  full_name: Carrión Vázquez, Mariano
  last_name: Carrión Vázquez
- first_name: Marek
  full_name: Cieplak, Marek
  last_name: Cieplak
citation:
  ama: 'Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M,
    Cieplak M. Theoretical tests of the mechanical protection strategy in protein
    nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. 2014;82(5):717-726.
    doi:<a href="https://doi.org/10.1002/prot.24436">10.1002/prot.24436</a>'
  apa: 'Chwastyk, M., Galera Prat, A., Sikora, M. K., Gómez Sicilia, À., Carrión Vázquez,
    M., &#38; Cieplak, M. (2014). Theoretical tests of the mechanical protection strategy
    in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>.
    Wiley-Blackwell. <a href="https://doi.org/10.1002/prot.24436">https://doi.org/10.1002/prot.24436</a>'
  chicago: 'Chwastyk, Mateusz, Albert Galera Prat, Mateusz K Sikora, Àngel Gómez Sicilia,
    Mariano Carrión Vázquez, and Marek Cieplak. “Theoretical Tests of the Mechanical
    Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function
    and Bioinformatics</i>. Wiley-Blackwell, 2014. <a href="https://doi.org/10.1002/prot.24436">https://doi.org/10.1002/prot.24436</a>.'
  ieee: 'M. Chwastyk, A. Galera Prat, M. K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez,
    and M. Cieplak, “Theoretical tests of the mechanical protection strategy in protein
    nanomechanics,” <i>Proteins: Structure, Function and Bioinformatics</i>, vol.
    82, no. 5. Wiley-Blackwell, pp. 717–726, 2014.'
  ista: 'Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M,
    Cieplak M. 2014. Theoretical tests of the mechanical protection strategy in protein
    nanomechanics. Proteins: Structure, Function and Bioinformatics. 82(5), 717–726.'
  mla: 'Chwastyk, Mateusz, et al. “Theoretical Tests of the Mechanical Protection
    Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>,
    vol. 82, no. 5, Wiley-Blackwell, 2014, pp. 717–26, doi:<a href="https://doi.org/10.1002/prot.24436">10.1002/prot.24436</a>.'
  short: 'M. Chwastyk, A. Galera Prat, M.K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez,
    M. Cieplak, Proteins: Structure, Function and Bioinformatics 82 (2014) 717–726.'
date_created: 2018-12-11T11:54:34Z
date_published: 2014-05-01T00:00:00Z
date_updated: 2025-09-29T13:06:49Z
day: '01'
department:
- _id: CaHe
doi: 10.1002/prot.24436
external_id:
  isi:
  - '000337280400003'
intvolume: '        82'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa_version: None
page: 717 - 726
publication: 'Proteins: Structure, Function and Bioinformatics'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5204'
scopus_import: '1'
status: public
title: Theoretical tests of the mechanical protection strategy in protein nanomechanics
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 82
year: '2014'
...