{"publication_status":"published","acknowledgement":"We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core Facility and Proteomics Core Facility of the Central European Institute of Technology (CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR); and (iii) Plant Sciences Core Facility for support with obtaining scientific data presented here. We acknowledge support from the project National Institute of Virology and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European Union - Next Generation EU. This work received funding from the Czech Science Foundation grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI” (no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship funded by Brno city municipality to M.H.","type":"journal_article","has_accepted_license":"1","pmid":1,"quality_controlled":"1","language":[{"iso":"eng"}],"article_processing_charge":"Yes","article_number":"eadk1954 ","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"publication":"Science Advances","abstract":[{"text":"The globally distributed marine alga Emiliania huxleyi has cooling effect on the Earth’s climate. The population density of E. huxleyi is restricted by Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite the impact of E. huxleyi viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from E. huxleyi cells, which enables the continuous release of virions by exocytosis.","lang":"eng"}],"_id":"18920","publisher":"American Association for the Advancement of Science","author":[{"first_name":"Miroslav","full_name":"Homola, Miroslav","last_name":"Homola"},{"first_name":"Renate Carina","id":"3b7984c9-17ff-11ed-b6fe-f943c4a5b626","last_name":"Büttner","full_name":"Büttner, Renate Carina"},{"last_name":"Füzik","full_name":"Füzik, Tibor","first_name":"Tibor"},{"full_name":"Křepelka, Pavel","last_name":"Křepelka","first_name":"Pavel"},{"first_name":"Radka","full_name":"Holbová, Radka","last_name":"Holbová"},{"full_name":"Nováček, Jiří","last_name":"Nováček","first_name":"Jiří"},{"first_name":"Marten L.","full_name":"Chaillet, Marten L.","last_name":"Chaillet"},{"last_name":"Žák","full_name":"Žák, Jakub","first_name":"Jakub"},{"first_name":"Danyil","last_name":"Grybchuk","full_name":"Grybchuk, Danyil"},{"first_name":"Friedrich","full_name":"Förster, Friedrich","last_name":"Förster"},{"first_name":"William H.","full_name":"Wilson, William H.","last_name":"Wilson"},{"first_name":"Declan C.","last_name":"Schroeder","full_name":"Schroeder, Declan C."},{"first_name":"Pavel","last_name":"Plevka","full_name":"Plevka, Pavel"}],"publication_identifier":{"eissn":["2375-2548"]},"issue":"15","title":"Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi","department":[{"_id":"EM-Fac"}],"intvolume":"        10","date_updated":"2025-04-09T11:55:28Z","OA_place":"publisher","volume":10,"doi":"10.1126/sciadv.adk1954","related_material":{"link":[{"relation":"software","url":" https://github.com/fuzikt/tomostarpy."}]},"file":[{"file_name":"2024_ScienceAdv_Homola.pdf","checksum":"291dd7ceccbe6bfd8e0a9157584f88e9","date_updated":"2025-01-27T14:40:08Z","creator":"dernst","file_id":"18921","access_level":"open_access","success":1,"date_created":"2025-01-27T14:40:08Z","content_type":"application/pdf","relation":"main_file","file_size":40623405}],"DOAJ_listed":"1","article_type":"original","date_created":"2025-01-27T14:32:34Z","oa_version":"Published Version","oa":1,"OA_type":"gold","scopus_import":"1","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["38598627"]},"status":"public","month":"04","day":"01","year":"2024","date_published":"2024-04-01T00:00:00Z","file_date_updated":"2025-01-27T14:40:08Z"}