{"OA_type":"gold","author":[{"last_name":"Knabl","full_name":"Knabl, Paul","first_name":"Paul"},{"last_name":"Schauer","full_name":"Schauer, Alexandra","first_name":"Alexandra","orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Pomreinke, Autumn P","last_name":"Pomreinke","first_name":"Autumn P"},{"first_name":"Bob","full_name":"Zimmermann, Bob","last_name":"Zimmermann"},{"first_name":"Katherine W","last_name":"Rogers","full_name":"Rogers, Katherine W"},{"first_name":"Daniel","last_name":"Čapek","full_name":"Čapek, Daniel"},{"first_name":"Patrick","last_name":"Müller","full_name":"Müller, Patrick"},{"last_name":"Genikhovich","full_name":"Genikhovich, Grigory","first_name":"Grigory"}],"date_updated":"2025-01-29T08:56:21Z","publication":"eLife","_id":"18940","type":"journal_article","article_type":"original","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"publication_status":"published","title":"Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator","date_published":"2024-02-07T00:00:00Z","abstract":[{"lang":"eng","text":"BMP signaling has a conserved function in patterning the dorsal-ventral body axis in Bilateria and the directive axis in anthozoan cnidarians. So far, cnidarian studies have focused on the role of different BMP signaling network components in regulating pSMAD1/5 gradient formation. Much less is known about the target genes downstream of BMP signaling. To address this, we generated a genome-wide list of direct pSMAD1/5 target genes in the anthozoan <jats:italic>Nematostella vectensis</jats:italic>, several of which were conserved in <jats:italic>Drosophila</jats:italic> and <jats:italic>Xenopus</jats:italic>. Our ChIP-seq analysis revealed that many of the regulatory molecules with documented bilaterally symmetric expression in <jats:italic>Nematostella</jats:italic> are directly controlled by BMP signaling. We identified several so far uncharacterized BMP-dependent transcription factors and signaling molecules, whose bilaterally symmetric expression may be indicative of their involvement in secondary axis patterning. One of these molecules is <jats:italic>zswim4-6</jats:italic>, which encodes a novel nuclear protein that can modulate the pSMAD1/5 gradient and potentially promote BMP-dependent gene repression."}],"has_accepted_license":"1","publication_identifier":{"issn":["2050-084X"]},"department":[{"_id":"CaHe"}],"volume":13,"quality_controlled":"1","day":"07","OA_place":"publisher","file_date_updated":"2025-01-29T08:50:18Z","scopus_import":"1","year":"2024","month":"02","intvolume":"        13","file":[{"creator":"dernst","success":1,"date_created":"2025-01-29T08:50:18Z","file_id":"18941","checksum":"24548a184215d3f4547bba535ccfd7b1","file_name":"2024_eLife_Knabl.pdf","file_size":11855972,"date_updated":"2025-01-29T08:50:18Z","relation":"main_file","access_level":"open_access","content_type":"application/pdf"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"DOAJ_listed":"1","article_processing_charge":"Yes","doi":"10.7554/elife.80803","status":"public","date_created":"2025-01-29T08:48:34Z","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"chicago":"Knabl, Paul, Alexandra Schauer, Autumn P Pomreinke, Bob Zimmermann, Katherine W Rogers, Daniel Čapek, Patrick Müller, and Grigory Genikhovich. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>.","ieee":"P. Knabl <i>et al.</i>, “Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator,” <i>eLife</i>, vol. 13. eLife Sciences Publications, 2024.","apa":"Knabl, P., Schauer, A., Pomreinke, A. P., Zimmermann, B., Rogers, K. W., Čapek, D., … Genikhovich, G. (2024). Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>","ama":"Knabl P, Schauer A, Pomreinke AP, et al. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>eLife</i>. 2024;13. doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>","mla":"Knabl, Paul, et al. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>, vol. 13, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>.","short":"P. Knabl, A. Schauer, A.P. Pomreinke, B. Zimmermann, K.W. Rogers, D. Čapek, P. Müller, G. Genikhovich, ELife 13 (2024).","ista":"Knabl P, Schauer A, Pomreinke AP, Zimmermann B, Rogers KW, Čapek D, Müller P, Genikhovich G. 2024. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. eLife. 13."},"publisher":"eLife Sciences Publications","acknowledgement":"This work was funded by the Austrian Science Foundation (FWF) grants P26962-B21 and P32705-B to GG and by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 637840 [QUANTPATTERN] and 863952 [ACE-OF-SPACE]) to PM. We thank Michaela Schwaiger, Taras Kreslavsky, Hiromi Tagoh, and Patricio Ferrer Murguia for their help with the ChIP protocol, Matthias Richter and Christian Hofer for their assistance with in situ analyses, Emilio Gonzalez Morales for making the measurements for Figure 6—figure supplement 3, Catrin Weiler for the assistance in cloning zebrafish zswim5, David Mörsdorf for critically reading the manuscript and help with data visualization, and the Core Facility for Cell Imaging and Ultrastructure Research of the University of Vienna for access to the confocal microscope."}