--- res: bibo_abstract: - Asymmetric cell divisions allow stem cells to balance proliferation and differentiation. During embryogenesis, murine epidermis expands rapidly from a single layer of unspecified basal layer progenitors to a stratified, differentiated epithelium. Morphogenesis involves perpendicular (asymmetric) divisions and the spindle orientation protein LGN, but little is known about how the apical localization of LGN is regulated. Here, we combine conventional genetics and lentiviral-mediated in vivo RNAi to explore the functions of the LGN-interacting proteins Par3, mInsc and Gα i3. Whereas loss of each gene alone leads to randomized division angles, combined loss of Gnai3 and mInsc causes a phenotype of mostly planar divisions, akin to loss of LGN. These findings lend experimental support for the hitherto untested model that Par3-mInsc and Gα i3 act cooperatively to polarize LGN and promote perpendicular divisions. Finally, we uncover a developmental switch between delamination-driven early stratification and spindle-orientation-dependent differentiation that occurs around E15, revealing a two-step mechanism underlying epidermal maturation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Scott foaf_name: Williams, Scott foaf_surname: Williams - foaf_Person: foaf_givenName: Lyndsay foaf_name: Ratliff, Lyndsay foaf_surname: Ratliff - foaf_Person: foaf_givenName: Maria P foaf_name: Postiglione, Maria P foaf_surname: Postiglione foaf_workInfoHomepage: http://www.librecat.org/personId=2C67902A-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Juergen foaf_name: Knoblich, Juergen foaf_surname: Knoblich - foaf_Person: foaf_givenName: Elaine foaf_name: Fuchs, Elaine foaf_surname: Fuchs bibo_doi: 10.1038/ncb3001 bibo_issue: '8' bibo_volume: 16 dct_date: 2014^xs_gYear dct_language: eng dct_publisher: Nature Publishing Group@ dct_title: Par3-mInsc and Gα i3 cooperate to promote oriented epidermal cell divisions through LGN@ fabio_hasPubmedId: '25016959' ...