---
_id: '1899'
abstract:
- lang: eng
  text: Asymmetric cell divisions allow stem cells to balance proliferation and differentiation.
    During embryogenesis, murine epidermis expands rapidly from a single layer of
    unspecified basal layer progenitors to a stratified, differentiated epithelium.
    Morphogenesis involves perpendicular (asymmetric) divisions and the spindle orientation
    protein LGN, but little is known about how the apical localization of LGN is regulated.
    Here, we combine conventional genetics and lentiviral-mediated in vivo RNAi to
    explore the functions of the LGN-interacting proteins Par3, mInsc and Gα i3. Whereas
    loss of each gene alone leads to randomized division angles, combined loss of
    Gnai3 and mInsc causes a phenotype of mostly planar divisions, akin to loss of
    LGN. These findings lend experimental support for the hitherto untested model
    that Par3-mInsc and Gα i3 act cooperatively to polarize LGN and promote perpendicular
    divisions. Finally, we uncover a developmental switch between delamination-driven
    early stratification and spindle-orientation-dependent differentiation that occurs
    around E15, revealing a two-step mechanism underlying epidermal maturation.
article_processing_charge: No
article_type: original
author:
- first_name: Scott
  full_name: Williams, Scott
  last_name: Williams
- first_name: Lyndsay
  full_name: Ratliff, Lyndsay
  last_name: Ratliff
- first_name: Maria P
  full_name: Postiglione, Maria P
  id: 2C67902A-F248-11E8-B48F-1D18A9856A87
  last_name: Postiglione
- first_name: Juergen
  full_name: Knoblich, Juergen
  last_name: Knoblich
- first_name: Elaine
  full_name: Fuchs, Elaine
  last_name: Fuchs
citation:
  ama: Williams S, Ratliff L, Postiglione MP, Knoblich J, Fuchs E. Par3-mInsc and
    Gα i3 cooperate to promote oriented epidermal cell divisions through LGN. <i>Nature
    Cell Biology</i>. 2014;16(8):758-769. doi:<a href="https://doi.org/10.1038/ncb3001">10.1038/ncb3001</a>
  apa: Williams, S., Ratliff, L., Postiglione, M. P., Knoblich, J., &#38; Fuchs, E.
    (2014). Par3-mInsc and Gα i3 cooperate to promote oriented epidermal cell divisions
    through LGN. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncb3001">https://doi.org/10.1038/ncb3001</a>
  chicago: Williams, Scott, Lyndsay Ratliff, Maria P Postiglione, Juergen Knoblich,
    and Elaine Fuchs. “Par3-MInsc and Gα I3 Cooperate to Promote Oriented Epidermal
    Cell Divisions through LGN.” <i>Nature Cell Biology</i>. Nature Publishing Group,
    2014. <a href="https://doi.org/10.1038/ncb3001">https://doi.org/10.1038/ncb3001</a>.
  ieee: S. Williams, L. Ratliff, M. P. Postiglione, J. Knoblich, and E. Fuchs, “Par3-mInsc
    and Gα i3 cooperate to promote oriented epidermal cell divisions through LGN,”
    <i>Nature Cell Biology</i>, vol. 16, no. 8. Nature Publishing Group, pp. 758–769,
    2014.
  ista: Williams S, Ratliff L, Postiglione MP, Knoblich J, Fuchs E. 2014. Par3-mInsc
    and Gα i3 cooperate to promote oriented epidermal cell divisions through LGN.
    Nature Cell Biology. 16(8), 758–769.
  mla: Williams, Scott, et al. “Par3-MInsc and Gα I3 Cooperate to Promote Oriented
    Epidermal Cell Divisions through LGN.” <i>Nature Cell Biology</i>, vol. 16, no.
    8, Nature Publishing Group, 2014, pp. 758–69, doi:<a href="https://doi.org/10.1038/ncb3001">10.1038/ncb3001</a>.
  short: S. Williams, L. Ratliff, M.P. Postiglione, J. Knoblich, E. Fuchs, Nature
    Cell Biology 16 (2014) 758–769.
date_created: 2018-12-11T11:54:36Z
date_published: 2014-07-13T00:00:00Z
date_updated: 2025-09-29T12:32:05Z
day: '13'
department:
- _id: SiHi
doi: 10.1038/ncb3001
external_id:
  isi:
  - '000339904900008'
  pmid:
  - '25016959'
intvolume: '        16'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159251/
month: '07'
oa: 1
oa_version: Submitted Version
page: 758 - 769
pmid: 1
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5196'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Par3-mInsc and Gα i3 cooperate to promote oriented epidermal cell divisions
  through LGN
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 16
year: '2014'
...