---
res:
  bibo_abstract:
  - In the 1960s-1980s, determination of bacterial growth rates was an important tool
    in microbial genetics, biochemistry, molecular biology, and microbial physiology.
    The exciting technical developments of the 1990s and the 2000s eclipsed that tool;
    as a result, many investigators today lack experience with growth rate measurements.
    Recently, investigators in a number of areas have started to use measurements
    of bacterial growth rates for a variety of purposes. Those measurements have been
    greatly facilitated by the availability of microwell plate readers that permit
    the simultaneous measurements on up to 384 different cultures. Only the exponential
    (logarithmic) portions of the resulting growth curves are useful for determining
    growth rates, and manual determination of that portion and calculation of growth
    rates can be tedious for high-throughput purposes. Here, we introduce the program
    GrowthRates that uses plate reader output files to automatically determine the
    exponential portion of the curve and to automatically calculate the growth rate,
    the maximum culture density, and the duration of the growth lag phase. GrowthRates
    is freely available for Macintosh, Windows, and Linux.We discuss the effects of
    culture volume, the classical bacterial growth curve, and the differences between
    determinations in rich media and minimal (mineral salts) media. This protocol
    covers calibration of the plate reader, growth of culture inocula for both rich
    and minimal media, and experimental setup. As a guide to reliability, we report
    typical day-to-day variation in growth rates and variation within experiments
    with respect to position of wells within the plates.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Barry
      foaf_name: Hall, Barry
      foaf_surname: Hall
  - foaf_Person:
      foaf_givenName: Hande
      foaf_name: Acar, Hande
      foaf_surname: Acar
      foaf_workInfoHomepage: http://www.librecat.org/personId=2DDF136A-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-1986-9753
  - foaf_Person:
      foaf_givenName: Anna
      foaf_name: Nandipati, Anna
      foaf_surname: Nandipati
  - foaf_Person:
      foaf_givenName: Miriam
      foaf_name: Barlow, Miriam
      foaf_surname: Barlow
  bibo_doi: 10.1093/molbev/mst187
  bibo_issue: '1'
  bibo_volume: 31
  dct_date: 2014^xs_gYear
  dct_identifier:
  - UT:000329253200022
  dct_isPartOf:
  - http://id.crossref.org/issn/0737-4038
  - http://id.crossref.org/issn/1537-1719
  dct_language: eng
  dct_publisher: Oxford University Press@
  dct_title: Growth rates made easy@
  fabio_hasPubmedId: '24170494'
...