---
res:
  bibo_abstract:
  - Pathogenic fungal and bacterial cells are enveloped within a cell wall, a molecular
    barrier at their cell surface, and a critical architecture that constantly evolves
    during pathogenesis. Understanding the molecular composition, structural organization,
    and mobility of polysaccharides constituting this cell envelope is crucial to
    correlate cell wall organization with its role in pathogenicity and to identify
    potential antifungal targets. For the fungal pathogen Cryptococcus neoformans,
    the characterization of the cell envelope has been complexified by the presence
    of an additional external polysaccharide capsular shell. Here, we investigate
    how magic-angle spinning (MAS) solid-state NMR techniques increase the analytical
    capabilities to characterize the structure and dynamics of this encapsulated pathogen.
    The versatility of proton detection experiments, dynamic-based filters, and relaxation
    measurements facilitate the discrimination of the highly mobile external capsular
    structure from the internal rigid cell wall of C. neoformans. In addition, we
    report the in situ detection of triglyceride molecules from lipid droplets based
    on NMR dynamic filters. Together, we demonstrate a nondestructive technique to
    study the cell wall architecture of encapsulated microbes using C. neoformans
    as a model, an airborne opportunistic fungal pathogen that infects mainly immunocompromised
    but also competent hosts.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Alons
      foaf_name: Lends, Alons
      foaf_surname: Lends
  - foaf_Person:
      foaf_givenName: Gaelle
      foaf_name: Lamon, Gaelle
      foaf_surname: Lamon
  - foaf_Person:
      foaf_givenName: Loic
      foaf_name: Delcourte, Loic
      foaf_surname: Delcourte
  - foaf_Person:
      foaf_givenName: Aude
      foaf_name: Sturny-Leclere, Aude
      foaf_surname: Sturny-Leclere
  - foaf_Person:
      foaf_givenName: Axelle
      foaf_name: Grélard, Axelle
      foaf_surname: Grélard
  - foaf_Person:
      foaf_givenName: Estelle
      foaf_name: Morvan, Estelle
      foaf_surname: Morvan
  - foaf_Person:
      foaf_givenName: Muhammed Bilal
      foaf_name: Abdul-Shukkoor, Muhammed Bilal
      foaf_surname: Abdul-Shukkoor
  - foaf_Person:
      foaf_givenName: Mélanie
      foaf_name: Berbon, Mélanie
      foaf_surname: Berbon
  - foaf_Person:
      foaf_givenName: Alicia
      foaf_name: Vallet, Alicia
      foaf_surname: Vallet
  - foaf_Person:
      foaf_givenName: Birgit
      foaf_name: Habenstein, Birgit
      foaf_surname: Habenstein
  - foaf_Person:
      foaf_givenName: Erick J.
      foaf_name: Dufourc, Erick J.
      foaf_surname: Dufourc
  - foaf_Person:
      foaf_givenName: Paul
      foaf_name: Schanda, Paul
      foaf_surname: Schanda
      foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425
    orcid: 0000-0002-9350-7606
  - foaf_Person:
      foaf_givenName: Vishukumar
      foaf_name: Aimanianda, Vishukumar
      foaf_surname: Aimanianda
  - foaf_Person:
      foaf_givenName: Antoine
      foaf_name: Loquet, Antoine
      foaf_surname: Loquet
  bibo_doi: 10.1021/jacs.4c16975
  bibo_issue: '8'
  bibo_volume: 147
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001423628600001
  dct_isPartOf:
  - http://id.crossref.org/issn/0002-7863
  - http://id.crossref.org/issn/1520-5126
  dct_language: eng
  dct_publisher: American Chemical Society@
  dct_title: Molecular distinction of cell wall and capsular polysaccharides in encapsulated
    pathogens by in situ magic-angle spinning NMR techniques@
  fabio_hasPubmedId: '39955787'
...