{"article_processing_charge":"No","publisher":"American Chemical Society","oa_version":"None","doi":"10.1021/jacs.4c16975","abstract":[{"lang":"eng","text":"Pathogenic fungal and bacterial cells are enveloped within a cell wall, a molecular barrier at their cell surface, and a critical architecture that constantly evolves during pathogenesis. Understanding the molecular composition, structural organization, and mobility of polysaccharides constituting this cell envelope is crucial to correlate cell wall organization with its role in pathogenicity and to identify potential antifungal targets. For the fungal pathogen Cryptococcus neoformans, the characterization of the cell envelope has been complexified by the presence of an additional external polysaccharide capsular shell. Here, we investigate how magic-angle spinning (MAS) solid-state NMR techniques increase the analytical capabilities to characterize the structure and dynamics of this encapsulated pathogen. The versatility of proton detection experiments, dynamic-based filters, and relaxation measurements facilitate the discrimination of the highly mobile external capsular structure from the internal rigid cell wall of C. neoformans. In addition, we report the in situ detection of triglyceride molecules from lipid droplets based on NMR dynamic filters. Together, we demonstrate a nondestructive technique to study the cell wall architecture of encapsulated microbes using C. neoformans as a model, an airborne opportunistic fungal pathogen that infects mainly immunocompromised but also competent hosts."}],"pmid":1,"OA_type":"closed access","date_published":"2025-02-16T00:00:00Z","year":"2025","author":[{"last_name":"Lends","full_name":"Lends, Alons","first_name":"Alons"},{"first_name":"Gaelle","full_name":"Lamon, Gaelle","last_name":"Lamon"},{"first_name":"Loic","full_name":"Delcourte, Loic","last_name":"Delcourte"},{"full_name":"Sturny-Leclere, Aude","last_name":"Sturny-Leclere","first_name":"Aude"},{"first_name":"Axelle","last_name":"Grélard","full_name":"Grélard, Axelle"},{"first_name":"Estelle","last_name":"Morvan","full_name":"Morvan, Estelle"},{"first_name":"Muhammed Bilal","last_name":"Abdul-Shukkoor","full_name":"Abdul-Shukkoor, Muhammed Bilal"},{"first_name":"Mélanie","full_name":"Berbon, Mélanie","last_name":"Berbon"},{"last_name":"Vallet","full_name":"Vallet, Alicia","first_name":"Alicia"},{"last_name":"Habenstein","full_name":"Habenstein, Birgit","first_name":"Birgit"},{"full_name":"Dufourc, Erick J.","last_name":"Dufourc","first_name":"Erick J."},{"last_name":"Schanda","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","orcid":"0000-0002-9350-7606","first_name":"Paul"},{"first_name":"Vishukumar","full_name":"Aimanianda, Vishukumar","last_name":"Aimanianda"},{"last_name":"Loquet","full_name":"Loquet, Antoine","first_name":"Antoine"}],"citation":{"ama":"Lends A, Lamon G, Delcourte L, et al. Molecular distinction of cell wall and capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning NMR techniques. <i>Journal of the American Chemical Society</i>. 2025;147(8):6813-6824. doi:<a href=\"https://doi.org/10.1021/jacs.4c16975\">10.1021/jacs.4c16975</a>","ieee":"A. Lends <i>et al.</i>, “Molecular distinction of cell wall and capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning NMR techniques,” <i>Journal of the American Chemical Society</i>, vol. 147, no. 8. American Chemical Society, pp. 6813–6824, 2025.","apa":"Lends, A., Lamon, G., Delcourte, L., Sturny-Leclere, A., Grélard, A., Morvan, E., … Loquet, A. (2025). Molecular distinction of cell wall and capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning NMR techniques. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.4c16975\">https://doi.org/10.1021/jacs.4c16975</a>","mla":"Lends, Alons, et al. “Molecular Distinction of Cell Wall and Capsular Polysaccharides in Encapsulated Pathogens by in Situ Magic-Angle Spinning NMR Techniques.” <i>Journal of the American Chemical Society</i>, vol. 147, no. 8, American Chemical Society, 2025, pp. 6813–24, doi:<a href=\"https://doi.org/10.1021/jacs.4c16975\">10.1021/jacs.4c16975</a>.","ista":"Lends A, Lamon G, Delcourte L, Sturny-Leclere A, Grélard A, Morvan E, Abdul-Shukkoor MB, Berbon M, Vallet A, Habenstein B, Dufourc EJ, Schanda P, Aimanianda V, Loquet A. 2025. Molecular distinction of cell wall and capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning NMR techniques. Journal of the American Chemical Society. 147(8), 6813–6824.","short":"A. Lends, G. Lamon, L. Delcourte, A. Sturny-Leclere, A. Grélard, E. Morvan, M.B. Abdul-Shukkoor, M. Berbon, A. Vallet, B. Habenstein, E.J. Dufourc, P. Schanda, V. Aimanianda, A. Loquet, Journal of the American Chemical Society 147 (2025) 6813–6824.","chicago":"Lends, Alons, Gaelle Lamon, Loic Delcourte, Aude Sturny-Leclere, Axelle Grélard, Estelle Morvan, Muhammed Bilal Abdul-Shukkoor, et al. “Molecular Distinction of Cell Wall and Capsular Polysaccharides in Encapsulated Pathogens by in Situ Magic-Angle Spinning NMR Techniques.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2025. <a href=\"https://doi.org/10.1021/jacs.4c16975\">https://doi.org/10.1021/jacs.4c16975</a>."},"intvolume":"       147","language":[{"iso":"eng"}],"status":"public","publication":"Journal of the American Chemical Society","title":"Molecular distinction of cell wall and capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning NMR techniques","_id":"19072","month":"02","scopus_import":"1","external_id":{"pmid":["39955787"],"isi":["001423628600001"]},"publication_identifier":{"eissn":["1520-5126"],"issn":["0002-7863"]},"acknowledgement":"We thank the ANR (ANR-16-CE11-0020-02 to A. Loquet, and V.A. and ANR-21-CE17-0032-01 grant FUNPOLYVAC to V.A.) as well as the Swiss National Science Foundation for early postdoc mobility project P2EZP2_184258 to A. Lends. This work has benefited from the Biophysical and Structural Chemistry Platform at Institut Européen de Chimie et Biologie IECB, Centre National de la Recherche Scientifique CNRS Unité d’Appui et de Recherche UAR 3033, INSERM US001, and CNRS (IR-RMN FR3050 and Infranalytics FR2054).","quality_controlled":"1","volume":147,"page":"6813-6824","department":[{"_id":"PaSc"}],"article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","issue":"8","day":"16","date_created":"2025-02-23T23:01:56Z","date_updated":"2025-09-30T10:36:53Z","isi":1,"type":"journal_article"}