---
OA_type: closed access
_id: '19072'
abstract:
- lang: eng
  text: Pathogenic fungal and bacterial cells are enveloped within a cell wall, a
    molecular barrier at their cell surface, and a critical architecture that constantly
    evolves during pathogenesis. Understanding the molecular composition, structural
    organization, and mobility of polysaccharides constituting this cell envelope
    is crucial to correlate cell wall organization with its role in pathogenicity
    and to identify potential antifungal targets. For the fungal pathogen Cryptococcus
    neoformans, the characterization of the cell envelope has been complexified by
    the presence of an additional external polysaccharide capsular shell. Here, we
    investigate how magic-angle spinning (MAS) solid-state NMR techniques increase
    the analytical capabilities to characterize the structure and dynamics of this
    encapsulated pathogen. The versatility of proton detection experiments, dynamic-based
    filters, and relaxation measurements facilitate the discrimination of the highly
    mobile external capsular structure from the internal rigid cell wall of C. neoformans.
    In addition, we report the in situ detection of triglyceride molecules from lipid
    droplets based on NMR dynamic filters. Together, we demonstrate a nondestructive
    technique to study the cell wall architecture of encapsulated microbes using C.
    neoformans as a model, an airborne opportunistic fungal pathogen that infects
    mainly immunocompromised but also competent hosts.
acknowledgement: We thank the ANR (ANR-16-CE11-0020-02 to A. Loquet, and V.A. and
  ANR-21-CE17-0032-01 grant FUNPOLYVAC to V.A.) as well as the Swiss National Science
  Foundation for early postdoc mobility project P2EZP2_184258 to A. Lends. This work
  has benefited from the Biophysical and Structural Chemistry Platform at Institut
  Européen de Chimie et Biologie IECB, Centre National de la Recherche Scientifique
  CNRS Unité d’Appui et de Recherche UAR 3033, INSERM US001, and CNRS (IR-RMN FR3050
  and Infranalytics FR2054).
article_processing_charge: No
article_type: original
author:
- first_name: Alons
  full_name: Lends, Alons
  last_name: Lends
- first_name: Gaelle
  full_name: Lamon, Gaelle
  last_name: Lamon
- first_name: Loic
  full_name: Delcourte, Loic
  last_name: Delcourte
- first_name: Aude
  full_name: Sturny-Leclere, Aude
  last_name: Sturny-Leclere
- first_name: Axelle
  full_name: Grélard, Axelle
  last_name: Grélard
- first_name: Estelle
  full_name: Morvan, Estelle
  last_name: Morvan
- first_name: Muhammed Bilal
  full_name: Abdul-Shukkoor, Muhammed Bilal
  last_name: Abdul-Shukkoor
- first_name: Mélanie
  full_name: Berbon, Mélanie
  last_name: Berbon
- first_name: Alicia
  full_name: Vallet, Alicia
  last_name: Vallet
- first_name: Birgit
  full_name: Habenstein, Birgit
  last_name: Habenstein
- first_name: Erick J.
  full_name: Dufourc, Erick J.
  last_name: Dufourc
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Vishukumar
  full_name: Aimanianda, Vishukumar
  last_name: Aimanianda
- first_name: Antoine
  full_name: Loquet, Antoine
  last_name: Loquet
citation:
  ama: Lends A, Lamon G, Delcourte L, et al. Molecular distinction of cell wall and
    capsular polysaccharides in encapsulated pathogens by in situ magic-angle spinning
    NMR techniques. <i>Journal of the American Chemical Society</i>. 2025;147(8):6813-6824.
    doi:<a href="https://doi.org/10.1021/jacs.4c16975">10.1021/jacs.4c16975</a>
  apa: Lends, A., Lamon, G., Delcourte, L., Sturny-Leclere, A., Grélard, A., Morvan,
    E., … Loquet, A. (2025). Molecular distinction of cell wall and capsular polysaccharides
    in encapsulated pathogens by in situ magic-angle spinning NMR techniques. <i>Journal
    of the American Chemical Society</i>. American Chemical Society. <a href="https://doi.org/10.1021/jacs.4c16975">https://doi.org/10.1021/jacs.4c16975</a>
  chicago: Lends, Alons, Gaelle Lamon, Loic Delcourte, Aude Sturny-Leclere, Axelle
    Grélard, Estelle Morvan, Muhammed Bilal Abdul-Shukkoor, et al. “Molecular Distinction
    of Cell Wall and Capsular Polysaccharides in Encapsulated Pathogens by in Situ
    Magic-Angle Spinning NMR Techniques.” <i>Journal of the American Chemical Society</i>.
    American Chemical Society, 2025. <a href="https://doi.org/10.1021/jacs.4c16975">https://doi.org/10.1021/jacs.4c16975</a>.
  ieee: A. Lends <i>et al.</i>, “Molecular distinction of cell wall and capsular polysaccharides
    in encapsulated pathogens by in situ magic-angle spinning NMR techniques,” <i>Journal
    of the American Chemical Society</i>, vol. 147, no. 8. American Chemical Society,
    pp. 6813–6824, 2025.
  ista: Lends A, Lamon G, Delcourte L, Sturny-Leclere A, Grélard A, Morvan E, Abdul-Shukkoor
    MB, Berbon M, Vallet A, Habenstein B, Dufourc EJ, Schanda P, Aimanianda V, Loquet
    A. 2025. Molecular distinction of cell wall and capsular polysaccharides in encapsulated
    pathogens by in situ magic-angle spinning NMR techniques. Journal of the American
    Chemical Society. 147(8), 6813–6824.
  mla: Lends, Alons, et al. “Molecular Distinction of Cell Wall and Capsular Polysaccharides
    in Encapsulated Pathogens by in Situ Magic-Angle Spinning NMR Techniques.” <i>Journal
    of the American Chemical Society</i>, vol. 147, no. 8, American Chemical Society,
    2025, pp. 6813–24, doi:<a href="https://doi.org/10.1021/jacs.4c16975">10.1021/jacs.4c16975</a>.
  short: A. Lends, G. Lamon, L. Delcourte, A. Sturny-Leclere, A. Grélard, E. Morvan,
    M.B. Abdul-Shukkoor, M. Berbon, A. Vallet, B. Habenstein, E.J. Dufourc, P. Schanda,
    V. Aimanianda, A. Loquet, Journal of the American Chemical Society 147 (2025)
    6813–6824.
date_created: 2025-02-23T23:01:56Z
date_published: 2025-02-16T00:00:00Z
date_updated: 2025-09-30T10:36:53Z
day: '16'
department:
- _id: PaSc
doi: 10.1021/jacs.4c16975
external_id:
  isi:
  - '001423628600001'
  pmid:
  - '39955787'
intvolume: '       147'
isi: 1
issue: '8'
language:
- iso: eng
month: '02'
oa_version: None
page: 6813-6824
pmid: 1
publication: Journal of the American Chemical Society
publication_identifier:
  eissn:
  - 1520-5126
  issn:
  - 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular distinction of cell wall and capsular polysaccharides in encapsulated
  pathogens by in situ magic-angle spinning NMR techniques
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 147
year: '2025'
...