Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling

article Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling published yes Tongda Xu author Ning Dai author Jisheng Chen author Shingo Nagawa author Min Cao author Hongjiang Li author 33CA54A6-F248-11E8-B48F-1D18A9856A870000-0001-5039-9660 Zimin Zhou author Xu Chen author 4E5ADCAA-F248-11E8-B48F-1D18A9856A87 Riet De Rycke author Hana Rakusová author Wen Wang author Alan Jones author Jirí Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 Sara Patterson author Anthony Bleecker author Zhenbiao Yang author JiFr department Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and was shown to be essential for plant development and morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 and transduce auxin signal to activate plasma membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading to changes in the cytoskeleton and the shape of leaf pavement cells in Arabidopsis. The interaction between ABP1 and TMK at the cell surface is induced by auxin and requires ABP1 sensing of auxin. These findings show that TMK proteins and ABP1 form a cell surface auxin perception complex that activates ROP signaling pathways, regulating nontranscriptional cytoplasmic responses and associated fundamental processes. American Association for the Advancement of Science2014 eng Science 24578577 00033230960004610.1126/science.1245125 34361741025 - 1028 Xu, Tongda, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” Science, vol. 343, no. 6174, American Association for the Advancement of Science, 2014, pp. 1025–28, doi:<a href="https://doi.org/10.1126/science.1245125">10.1126/science.1245125</a>. Xu, Tongda, Ning Dai, Jisheng Chen, Shingo Nagawa, Min Cao, Hongjiang Li, Zimin Zhou, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” Science. American Association for the Advancement of Science, 2014. <a href="https://doi.org/10.1126/science.1245125">https://doi.org/10.1126/science.1245125</a>. Xu T, Dai N, Chen J, Nagawa S, Cao M, Li H, Zhou Z, Chen X, De Rycke R, Rakusová H, Wang W, Jones A, Friml J, Patterson S, Bleecker A, Yang Z. 2014. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. 343(6174), 1025–1028. Xu, T., Dai, N., Chen, J., Nagawa, S., Cao, M., Li, H., … Yang, Z. (2014). Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.1245125">https://doi.org/10.1126/science.1245125</a> Xu T, Dai N, Chen J, et al. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. 2014;343(6174):1025-1028. doi:<a href="https://doi.org/10.1126/science.1245125">10.1126/science.1245125</a> T. Xu, N. Dai, J. Chen, S. Nagawa, M. Cao, H. Li, Z. Zhou, X. Chen, R. De Rycke, H. Rakusová, W. Wang, A. Jones, J. Friml, S. Patterson, A. Bleecker, Z. Yang, Science 343 (2014) 1025–1028. T. Xu et al., “Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling,” Science, vol. 343, no. 6174. American Association for the Advancement of Science, pp. 1025–1028, 2014. 19172018-12-11T11:54:42Z2025-09-29T12:20:10Z