---
_id: '1925'
abstract:
- lang: eng
  text: In the past decade carbon nanotubes (CNTs) have been widely studied as a potential
    drug-delivery system, especially with functionality for cellular targeting. Yet,
    little is known about the actual process of docking to cell receptors and transport
    dynamics after internalization. Here we performed single-particle studies of folic
    acid (FA) mediated CNT binding to human carcinoma cells and their transport inside
    the cytosol. In particular, we employed molecular recognition force spectroscopy,
    an atomic force microscopy based method, to visualize and quantify docking of
    FA functionalized CNTs to FA binding receptors in terms of binding probability
    and binding force. We then traced individual fluorescently labeled, FA functionalized
    CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed
    trajectories of directed diffusion and areas of nanotube confinement in the cytosol.
    Our results demonstrate the potential of a single-molecule approach for investigation
    of drug-delivery vehicles and their targeting capacity.
acknowledgement: "This work was supported by EC grant Marie Curie RTN-CT-2006-035616,
  CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net
  823980, 'IntelliTip'.\r\n"
article_number: '125704'
article_processing_charge: No
article_type: original
author:
- first_name: Constanze
  full_name: Lamprecht, Constanze
  last_name: Lamprecht
- first_name: Birgit
  full_name: Plochberger, Birgit
  last_name: Plochberger
- first_name: Verena
  full_name: Ruprecht, Verena
  id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
  last_name: Ruprecht
  orcid: 0000-0003-4088-8633
- first_name: Stefan
  full_name: Wieser, Stefan
  id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
  last_name: Wieser
  orcid: 0000-0002-2670-2217
- first_name: Christian
  full_name: Rankl, Christian
  last_name: Rankl
- first_name: Elena
  full_name: Heister, Elena
  last_name: Heister
- first_name: Barbara
  full_name: Unterauer, Barbara
  last_name: Unterauer
- first_name: Mario
  full_name: Brameshuber, Mario
  last_name: Brameshuber
- first_name: Jürgen
  full_name: Danzberger, Jürgen
  last_name: Danzberger
- first_name: Petar
  full_name: Lukanov, Petar
  last_name: Lukanov
- first_name: Emmanuel
  full_name: Flahaut, Emmanuel
  last_name: Flahaut
- first_name: Gerhard
  full_name: Schütz, Gerhard
  last_name: Schütz
- first_name: Peter
  full_name: Hinterdorfer, Peter
  last_name: Hinterdorfer
- first_name: Andreas
  full_name: Ebner, Andreas
  last_name: Ebner
citation:
  ama: Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to
    explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>.
    2014;25(12). doi:<a href="https://doi.org/10.1088/0957-4484/25/12/125704">10.1088/0957-4484/25/12/125704</a>
  apa: Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister,
    E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and
    transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing.
    <a href="https://doi.org/10.1088/0957-4484/25/12/125704">https://doi.org/10.1088/0957-4484/25/12/125704</a>
  chicago: Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser,
    Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach
    to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>.
    IOP Publishing, 2014. <a href="https://doi.org/10.1088/0957-4484/25/12/125704">https://doi.org/10.1088/0957-4484/25/12/125704</a>.
  ieee: C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding
    uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>,
    vol. 25, no. 12. IOP Publishing, 2014.
  ista: Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer
    B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P,
    Ebner A. 2014. A single-molecule approach to explore binding uptake and transport
    of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.
  mla: Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding
    Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>,
    vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href="https://doi.org/10.1088/0957-4484/25/12/125704">10.1088/0957-4484/25/12/125704</a>.
  short: C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister,
    B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz,
    P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).
date_created: 2018-12-11T11:54:45Z
date_published: 2014-03-28T00:00:00Z
date_updated: 2025-09-29T12:14:47Z
day: '28'
ddc:
- '570'
department:
- _id: CaHe
- _id: MiSi
doi: 10.1088/0957-4484/25/12/125704
external_id:
  isi:
  - '000332669300017'
file:
- access_level: open_access
  checksum: df4e03d225a19179e7790f6d87a12332
  content_type: application/pdf
  creator: dernst
  date_created: 2020-05-15T09:21:19Z
  date_updated: 2020-07-14T12:45:21Z
  file_id: '7856'
  file_name: 2014_Nanotechnology_Lamprecht.pdf
  file_size: 3804152
  relation: main_file
file_date_updated: 2020-07-14T12:45:21Z
has_accepted_license: '1'
intvolume: '        25'
isi: 1
issue: '12'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
publication: Nanotechnology
publication_status: published
publisher: IOP Publishing
publist_id: '5169'
scopus_import: '1'
status: public
title: A single-molecule approach to explore binding uptake and transport of cancer
  cell targeting nanotubes
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 25
year: '2014'
...