---
OA_place: repository
OA_type: gold
_id: '19294'
abstract:
- lang: eng
text: Active regulation of gene expression, orchestrated by complex interactions
of activators and repressors at promoters, controls the fate of organisms. In
contrast, basal expression at uninduced promoters is considered to be a dynamically
inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional
regulation. Here, we investigate the basal expression mode of the mar operon,
the main regulator of intrinsic multiple antibiotic resistance in Escherichia
coli, and link its dynamic properties to the non-canonical, yet highly conserved
start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements
across tens of generations reveal that basal expression consists of rare stochastic
gene expression pulses, which maximize variability in wildtype and, surprisingly,
transiently accelerate cellular elongation rates. Competition experiments show
that basal expression confers fitness advantages to wildtype across several transitions
between exponential and stationary growth by shortening lag times. The dynamically
rich basal expression of the mar operon has likely been evolutionarily maintained
for its role in growth homeostasis of Enterobacteria within the gut environment,
thereby allowing other ancillary gene regulatory roles to evolve, e.g. control
of costly-to-induce multi-drug efflux pumps. Understanding the complex selection
forces governing genetic systems involved in intrinsic multi-drug resistance is
crucial for effective public health measures.
article_processing_charge: No
author:
- first_name: Kirti
full_name: Jain, Kirti
id: 330F0278-F248-11E8-B48F-1D18A9856A87
last_name: Jain
orcid: 0000-0002-3809-0449
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Roderich
full_name: Römhild, Roderich
id: 68E56E44-62B0-11EA-B963-444F3DDC885E
last_name: Römhild
orcid: 0000-0001-9480-5261
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile
basal gene expression as a fitness determinant in bacteria.” 2025. doi:10.15479/AT:ISTA:19294
apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet,
C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant
in bacteria.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:19294
chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper
Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025.
https://doi.org/10.15479/AT:ISTA:19294.
ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet,
“Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’”
Institute of Science and Technology Austria, 2025.
ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data
for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:19294.
mla: Jain, Kirti, et al. Data for “Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.” Institute of Science and Technology Austria, 2025,
doi:10.15479/AT:ISTA:19294.
short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).
corr_author: '1'
date_created: 2025-03-04T13:27:21Z
date_published: 2025-03-04T00:00:00Z
date_updated: 2026-05-20T08:33:07Z
day: '04'
ddc:
- '570'
department:
- _id: CaGu
- _id: Bio
- _id: FyKo
- _id: GaTk
doi: 10.15479/AT:ISTA:19294
file:
- access_level: open_access
checksum: 11a5bab307a4e1e1598a1577d8a2fbb5
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19295'
file_name: Data1.xlsx
file_size: 269054
relation: main_file
success: 1
- access_level: open_access
checksum: 3b057894322639f0c1e11fb2e84173e6
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19296'
file_name: Data2.xlsx
file_size: 87143
relation: main_file
success: 1
- access_level: open_access
checksum: a551e1b79a138bb97ab96979aa475b3c
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19297'
file_name: Data3.xlsx
file_size: 129101
relation: main_file
success: 1
- access_level: open_access
checksum: d6909c9bf111f859058082b1a2f970c4
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19298'
file_name: Data4.xlsx
file_size: 86243
relation: main_file
success: 1
- access_level: open_access
checksum: e5725a3a118a3f06846104906c8792c7
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19299'
file_name: Data5.xlsx
file_size: 26049
relation: main_file
success: 1
- access_level: open_access
checksum: 16763c127049f14bd587dc885677dce1
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: dernst
date_created: 2025-03-04T13:08:52Z
date_updated: 2025-03-04T13:08:52Z
file_id: '19300'
file_name: RawData_2_3.xlsx
file_size: 7327253
relation: main_file
success: 1
- access_level: open_access
checksum: 2f3e1a368b4e3abc46bf37e02724f0f4
content_type: text/plain
creator: dernst
date_created: 2025-03-05T07:39:38Z
date_updated: 2025-03-05T07:39:38Z
file_id: '19301'
file_name: Readme.txt
file_size: 606
relation: main_file
success: 1
file_date_updated: 2025-03-05T07:39:38Z
has_accepted_license: '1'
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '19626'
relation: used_in_publication
status: public
status: public
title: Data for "Pulsatile basal gene expression as a fitness determinant in bacteria"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...