{"article_type":"original","department":[{"_id":"XiFe"}],"page":"176-191","article_processing_charge":"No","issue":"1","_id":"19406","publication_status":"published","abstract":[{"lang":"eng","text":"Polyploidization is a common occurrence in the evolutionary history of flowering plants, significantly contributing to their adaptability and diversity. However, the molecular mechanisms behind these adaptive advantages are not well understood.\r\nThrough comprehensive phenotyping of diploid and tetraploid clones from Citrus and Poncirus genera, we discovered that genome doubling significantly enhances salt stress resilience. Epigenetic and transcriptomic analyses revealed that increased ethylene production in the roots of tetraploid plants was associated with hypomethylation and enhanced chromatin accessibility of the ACO1 gene. This increased ethylene production activates the transcription of reactive oxygen species scavenging genes and stress-related hormone biosynthesis genes. Consequently, tetraploid plants exhibited superior root functionality under salt stress, maintaining improved cytosolic K+/Na+ homeostasis.\r\nTo genetically validate the link between salt stress resilience and ACO1 expression, we generated overexpression and knockout lines, confirming the central role of ACO1 expression regulation following genome doubling in salt stress resilience.\r\nOur work elucidates the molecular mechanisms underlying the role of genome doubling in stress resilience. We also highlight the importance of chromatin dynamics in fine-tuning ethylene gene expression and activating salt stress resilience pathways, offering valuable insights into plant adaptation and crop genome evolution."}],"external_id":{"pmid":["39969116"]},"language":[{"iso":"eng"}],"year":"2025","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"day":"01","quality_controlled":"1","scopus_import":"1","author":[{"first_name":"Xin","last_name":"Song","full_name":"Song, Xin"},{"full_name":"Zhang, Miao","last_name":"Zhang","first_name":"Miao"},{"first_name":"Ting Ting","last_name":"Wang","full_name":"Wang, Ting Ting"},{"first_name":"Yao Yuan","full_name":"Duan, Yao Yuan","last_name":"Duan"},{"full_name":"Ren, Jie","last_name":"Ren","first_name":"Jie"},{"first_name":"Hu","last_name":"Gao","full_name":"Gao, Hu"},{"last_name":"Fan","full_name":"Fan, Yan Jie","first_name":"Yan Jie"},{"full_name":"Xia, Qiang Ming","last_name":"Xia","first_name":"Qiang Ming"},{"first_name":"Hui Xiang","last_name":"Cao","full_name":"Cao, Hui Xiang"},{"last_name":"Xie","full_name":"Xie, Kai Dong","first_name":"Kai Dong"},{"last_name":"Wu","full_name":"Wu, Xiao Meng","first_name":"Xiao Meng"},{"first_name":"Fei","full_name":"Zhang, Fei","last_name":"Zhang"},{"full_name":"Zhang, Si Qi","last_name":"Zhang","first_name":"Si Qi"},{"last_name":"Huang","full_name":"Huang, Ying","id":"11b5bbff-8b61-11ed-b69e-d8ddd6bce951","first_name":"Ying"},{"last_name":"Boualem","full_name":"Boualem, Adnane","first_name":"Adnane"},{"first_name":"Abdelhafid","full_name":"Bendahmane, Abdelhafid","last_name":"Bendahmane"},{"first_name":"Feng Quan","full_name":"Tan, Feng Quan","last_name":"Tan"},{"first_name":"Wen Wu","full_name":"Guo, Wen Wu","last_name":"Guo"}],"publication":"New Phytologist","OA_type":"closed access","status":"public","doi":"10.1111/nph.20428","date_updated":"2025-03-17T09:48:36Z","pmid":1,"month":"04","acknowledgement":"We thank Prof. Qi Xie from the Institute of Genetics and Development, Chinese Academy of Sciences, for providing the YAO promoter-driven CRISPR/Cas9 vector, our colleague Dr Robert M. Larkin from Huazhong Agricultural University, and Dr Olivier Martin from IPS2 (INRAE, France) for critical reading of the manuscript. This research was financially supported by grants from the National Key Research & Development Program of China (2024YFD1200501), the National Natural Science Foundation of China (32172525 and 32202432), the Foundation of Hubei Hongshan laboratory (2021hszd009), the China Agricultural Research System (CARS-26) and the Department of Science and Technology of Hubei Province (2022BBA0019). A. Bendahmane is funded by the ANR BioAdapt (ANR-21-LCV3-0003), LabEx Saclay Plant Sciences (SPS) (ANR-10-LABX-40-SPS), and the NectarGland ERC Project (101095736).","date_created":"2025-03-16T23:01:25Z","publisher":"Wiley","type":"journal_article","intvolume":"       246","date_published":"2025-04-01T00:00:00Z","title":"Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"X. Song <i>et al.</i>, “Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants,” <i>New Phytologist</i>, vol. 246, no. 1. Wiley, pp. 176–191, 2025.","ama":"Song X, Zhang M, Wang TT, et al. Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. <i>New Phytologist</i>. 2025;246(1):176-191. doi:<a href=\"https://doi.org/10.1111/nph.20428\">10.1111/nph.20428</a>","apa":"Song, X., Zhang, M., Wang, T. T., Duan, Y. Y., Ren, J., Gao, H., … Guo, W. W. (2025). Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.20428\">https://doi.org/10.1111/nph.20428</a>","mla":"Song, Xin, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene Signaling in Citrus Plants.” <i>New Phytologist</i>, vol. 246, no. 1, Wiley, 2025, pp. 176–91, doi:<a href=\"https://doi.org/10.1111/nph.20428\">10.1111/nph.20428</a>.","ista":"Song X, Zhang M, Wang TT, Duan YY, Ren J, Gao H, Fan YJ, Xia QM, Cao HX, Xie KD, Wu XM, Zhang F, Zhang SQ, Huang Y, Boualem A, Bendahmane A, Tan FQ, Guo WW. 2025. Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. New Phytologist. 246(1), 176–191.","chicago":"Song, Xin, Miao Zhang, Ting Ting Wang, Yao Yuan Duan, Jie Ren, Hu Gao, Yan Jie Fan, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene Signaling in Citrus Plants.” <i>New Phytologist</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/nph.20428\">https://doi.org/10.1111/nph.20428</a>.","short":"X. Song, M. Zhang, T.T. Wang, Y.Y. Duan, J. Ren, H. Gao, Y.J. Fan, Q.M. Xia, H.X. Cao, K.D. Xie, X.M. Wu, F. Zhang, S.Q. Zhang, Y. Huang, A. Boualem, A. Bendahmane, F.Q. Tan, W.W. Guo, New Phytologist 246 (2025) 176–191."},"volume":246,"oa_version":"None"}