---
OA_type: closed access
_id: '19406'
abstract:
- lang: eng
text: "Polyploidization is a common occurrence in the evolutionary history of flowering
plants, significantly contributing to their adaptability and diversity. However,
the molecular mechanisms behind these adaptive advantages are not well understood.\r\nThrough
comprehensive phenotyping of diploid and tetraploid clones from Citrus and Poncirus
genera, we discovered that genome doubling significantly enhances salt stress
resilience. Epigenetic and transcriptomic analyses revealed that increased ethylene
production in the roots of tetraploid plants was associated with hypomethylation
and enhanced chromatin accessibility of the ACO1 gene. This increased ethylene
production activates the transcription of reactive oxygen species scavenging genes
and stress-related hormone biosynthesis genes. Consequently, tetraploid plants
exhibited superior root functionality under salt stress, maintaining improved
cytosolic K+/Na+ homeostasis.\r\nTo genetically validate the link between salt
stress resilience and ACO1 expression, we generated overexpression and knockout
lines, confirming the central role of ACO1 expression regulation following genome
doubling in salt stress resilience.\r\nOur work elucidates the molecular mechanisms
underlying the role of genome doubling in stress resilience. We also highlight
the importance of chromatin dynamics in fine-tuning ethylene gene expression and
activating salt stress resilience pathways, offering valuable insights into plant
adaptation and crop genome evolution."
acknowledgement: We thank Prof. Qi Xie from the Institute of Genetics and Development,
Chinese Academy of Sciences, for providing the YAO promoter-driven CRISPR/Cas9 vector,
our colleague Dr Robert M. Larkin from Huazhong Agricultural University, and Dr
Olivier Martin from IPS2 (INRAE, France) for critical reading of the manuscript.
This research was financially supported by grants from the National Key Research
& Development Program of China (2024YFD1200501), the National Natural Science Foundation
of China (32172525 and 32202432), the Foundation of Hubei Hongshan laboratory (2021hszd009),
the China Agricultural Research System (CARS-26) and the Department of Science and
Technology of Hubei Province (2022BBA0019). A. Bendahmane is funded by the ANR BioAdapt
(ANR-21-LCV3-0003), LabEx Saclay Plant Sciences (SPS) (ANR-10-LABX-40-SPS), and
the NectarGland ERC Project (101095736).
article_processing_charge: No
article_type: original
author:
- first_name: Xin
full_name: Song, Xin
last_name: Song
- first_name: Miao
full_name: Zhang, Miao
last_name: Zhang
- first_name: Ting Ting
full_name: Wang, Ting Ting
last_name: Wang
- first_name: Yao Yuan
full_name: Duan, Yao Yuan
last_name: Duan
- first_name: Jie
full_name: Ren, Jie
last_name: Ren
- first_name: Hu
full_name: Gao, Hu
last_name: Gao
- first_name: Yan Jie
full_name: Fan, Yan Jie
last_name: Fan
- first_name: Qiang Ming
full_name: Xia, Qiang Ming
last_name: Xia
- first_name: Hui Xiang
full_name: Cao, Hui Xiang
last_name: Cao
- first_name: Kai Dong
full_name: Xie, Kai Dong
last_name: Xie
- first_name: Xiao Meng
full_name: Wu, Xiao Meng
last_name: Wu
- first_name: Fei
full_name: Zhang, Fei
last_name: Zhang
- first_name: Si Qi
full_name: Zhang, Si Qi
last_name: Zhang
- first_name: Ying
full_name: Huang, Ying
id: 11b5bbff-8b61-11ed-b69e-d8ddd6bce951
last_name: Huang
- first_name: Adnane
full_name: Boualem, Adnane
last_name: Boualem
- first_name: Abdelhafid
full_name: Bendahmane, Abdelhafid
last_name: Bendahmane
- first_name: Feng Quan
full_name: Tan, Feng Quan
last_name: Tan
- first_name: Wen Wu
full_name: Guo, Wen Wu
last_name: Guo
citation:
ama: Song X, Zhang M, Wang TT, et al. Polyploidization leads to salt stress resilience
via ethylene signaling in citrus plants. New Phytologist. 2025;246(1):176-191.
doi:10.1111/nph.20428
apa: Song, X., Zhang, M., Wang, T. T., Duan, Y. Y., Ren, J., Gao, H., … Guo, W.
W. (2025). Polyploidization leads to salt stress resilience via ethylene signaling
in citrus plants. New Phytologist. Wiley. https://doi.org/10.1111/nph.20428
chicago: Song, Xin, Miao Zhang, Ting Ting Wang, Yao Yuan Duan, Jie Ren, Hu Gao,
Yan Jie Fan, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene
Signaling in Citrus Plants.” New Phytologist. Wiley, 2025. https://doi.org/10.1111/nph.20428.
ieee: X. Song et al., “Polyploidization leads to salt stress resilience via
ethylene signaling in citrus plants,” New Phytologist, vol. 246, no. 1.
Wiley, pp. 176–191, 2025.
ista: Song X, Zhang M, Wang TT, Duan YY, Ren J, Gao H, Fan YJ, Xia QM, Cao HX, Xie
KD, Wu XM, Zhang F, Zhang SQ, Huang Y, Boualem A, Bendahmane A, Tan FQ, Guo WW.
2025. Polyploidization leads to salt stress resilience via ethylene signaling
in citrus plants. New Phytologist. 246(1), 176–191.
mla: Song, Xin, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene
Signaling in Citrus Plants.” New Phytologist, vol. 246, no. 1, Wiley, 2025,
pp. 176–91, doi:10.1111/nph.20428.
short: X. Song, M. Zhang, T.T. Wang, Y.Y. Duan, J. Ren, H. Gao, Y.J. Fan, Q.M. Xia,
H.X. Cao, K.D. Xie, X.M. Wu, F. Zhang, S.Q. Zhang, Y. Huang, A. Boualem, A. Bendahmane,
F.Q. Tan, W.W. Guo, New Phytologist 246 (2025) 176–191.
date_created: 2025-03-16T23:01:25Z
date_published: 2025-04-01T00:00:00Z
date_updated: 2025-09-30T11:00:06Z
day: '01'
department:
- _id: XiFe
doi: 10.1111/nph.20428
external_id:
isi:
- '001424915600001'
pmid:
- '39969116'
intvolume: ' 246'
isi: 1
issue: '1'
language:
- iso: eng
month: '04'
oa_version: None
page: 176-191
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Polyploidization leads to salt stress resilience via ethylene signaling in
citrus plants
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 246
year: '2025'
...