{"quality_controlled":"1","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"}],"year":"2025","citation":{"short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. 2025. doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>","apa":"Tang, H., Chen, L., &#38; Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>, pcaf008, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>.","ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025.","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>."},"ec_funded":1,"day":"18","type":"journal_article","oa":1,"doi":"10.1093/pcp/pcaf008","publication":"Plant and Cell Physiology","abstract":[{"text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species.","lang":"eng"}],"date_published":"2025-01-18T00:00:00Z","external_id":{"pmid":["39829340"]},"article_type":"original","article_number":"pcaf008","corr_author":"1","publisher":"Oxford University Press","_id":"19420","title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","publication_identifier":{"issn":["0032-0781","1471-9053"]},"author":[{"last_name":"Tang","full_name":"Tang, Han","orcid":"0000-0001-6152-6637","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","first_name":"Han"},{"first_name":"L","last_name":"Chen","full_name":"Chen, L"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","department":[{"_id":"JiFr"}],"publication_status":"epub_ahead","scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1093/pcp/pcaf008"}],"article_processing_charge":"No","month":"01","language":[{"iso":"eng"}],"pmid":1,"date_created":"2025-03-19T09:44:19Z","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_type":"closed access","status":"public","date_updated":"2025-03-20T09:56:54Z"}