---
OA_type: closed access
_id: '19420'
abstract:
- lang: eng
  text: Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and
    regeneration in flowering plants. To gain insight into the evolution of this mechanism,
    we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte
    known for its remarkable cell reprogramming capacity. We used various approaches
    to manipulate auxin levels, including exogenous application, pharmacological manipulations,
    and auxin biosynthesis mutants. We observed no significant effect on the rate
    of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease
    in auxin levels upon excision, which was followed by a local increase before the
    reprogramming process began. Mutant analysis revealed that PpPINs are required
    for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates
    polarly at sites that will develop into future filamentous stem cells. In addition,
    hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a
    marked delay in reprogramming, whereas endogenous or nonpolar versions do not
    have this effect. These results underscore that both the levels and the polarity
    of PpPINA are important for efficient cell reprogramming. Overall, these findings
    highlight the pivotal role of PIN polarity in plant regeneration. Furthermore,
    they suggest that understanding polarity mechanisms could have broader implications
    for improving regenerative processes across various plant species.
acknowledgement: "The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time
  and efforts in critical reading and constructive advice on the manuscript. The authors
  gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants
  to support this study.\r\nThis work was supported by the European Research Council
  Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and
  Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.)."
article_number: pcaf008
article_processing_charge: No
article_type: original
author:
- first_name: Han
  full_name: Tang, Han
  id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
  last_name: Tang
  orcid: 0000-0001-6152-6637
- first_name: L
  full_name: Chen, L
  last_name: Chen
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf
    cell reprogramming. <i>Plant and Cell Physiology</i>. 2025. doi:<a href="https://doi.org/10.1093/pcp/pcaf008">10.1093/pcp/pcaf008</a>
  apa: Tang, H., Chen, L., &#38; Friml, J. (2025). Auxin fluctuation and PIN polarization
    in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/pcp/pcaf008">https://doi.org/10.1093/pcp/pcaf008</a>
  chicago: Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization
    in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>. Oxford University
    Press, 2025. <a href="https://doi.org/10.1093/pcp/pcaf008">https://doi.org/10.1093/pcp/pcaf008</a>.
  ieee: H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in
    moss leaf cell reprogramming.,” <i>Plant and Cell Physiology</i>. Oxford University
    Press, 2025.
  ista: Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss
    leaf cell reprogramming. Plant and Cell Physiology., pcaf008.
  mla: Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell
    Reprogramming.” <i>Plant and Cell Physiology</i>, pcaf008, Oxford University Press,
    2025, doi:<a href="https://doi.org/10.1093/pcp/pcaf008">10.1093/pcp/pcaf008</a>.
  short: H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).
corr_author: '1'
date_created: 2025-03-19T09:44:19Z
date_published: 2025-03-05T00:00:00Z
date_updated: 2025-09-30T11:05:55Z
day: '05'
department:
- _id: JiFr
doi: 10.1093/pcp/pcaf008
ec_funded: 1
external_id:
  isi:
  - '001436802900001'
  pmid:
  - '39829340'
fulldoi: https://doi.org/10.1093/pcp/pcaf008
isi: 1
language:
- iso: eng
month: '03'
oa_version: None
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant and Cell Physiology
publication_identifier:
  eissn:
  - 1471-9053
  issn:
  - 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2025'
...