{"oa":1,"publication":"New Phytologist","doi":"10.1111/nph.70019","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"quality_controlled":"1","year":"2025","citation":{"apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>.","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist.","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>.","ieee":"K. Kurtović <i>et al.</i>, “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” <i>New Phytologist</i>. Wiley, 2025.","short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist (2025).","ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. 2025. doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>"},"day":"06","type":"journal_article","OA_place":"publisher","article_type":"original","external_id":{"pmid":["40047465"]},"abstract":[{"text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.","lang":"eng"}],"date_published":"2025-03-06T00:00:00Z","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","publisher":"Wiley","_id":"19423","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","department":[{"_id":"JiFr"}],"publication_status":"epub_ahead","author":[{"first_name":"K","last_name":"Kurtović","full_name":"Kurtović, K"},{"first_name":"S","full_name":"Vosolsobě, S","last_name":"Vosolsobě"},{"first_name":"D","full_name":"Nedvěd, D","last_name":"Nedvěd"},{"first_name":"K","last_name":"Müller","full_name":"Müller, K"},{"first_name":"PI","last_name":"Dobrev","full_name":"Dobrev, PI"},{"full_name":"Schmidt, V","last_name":"Schmidt","first_name":"V"},{"last_name":"Piszczek","full_name":"Piszczek, P","first_name":"P"},{"full_name":"Kuhn, A","last_name":"Kuhn","first_name":"A"},{"first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana","last_name":"Smoljan"},{"last_name":"Fisher","full_name":"Fisher, TJ","first_name":"TJ"},{"last_name":"Weijers","full_name":"Weijers, D","first_name":"D"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Bowman, JL","last_name":"Bowman","first_name":"JL"},{"first_name":"J","last_name":"Petrášek","full_name":"Petrášek, J"}],"publication_identifier":{"issn":["1469-8137"]},"main_file_link":[{"url":"https://doi.org/10.1111/nph.70019","open_access":"1"}],"article_processing_charge":"Yes (via OA deal)","scopus_import":"1","pmid":1,"month":"03","language":[{"iso":"eng"}],"status":"public","date_updated":"2025-03-20T10:35:43Z","date_created":"2025-03-19T09:45:11Z","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_type":"hybrid"}