---
OA_place: publisher
OA_type: hybrid
_id: '19423'
abstract:
- lang: eng
  text: "Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic
    roles in land plants, but its function and transport mechanisms in algae remain
    poorly understood. We therefore aimed to explore the role of IAA in a complex,
    streptophyte algae Chara braunii.\r\nHere, we described novel responses of C.
    braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa
    and CbPINc. We determined their localization in C. braunii using epitope-specific
    antibodies and tested their function in heterologous land plant models. Further,
    using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe
    thallus regeneration assay showed that IAA promotes thallus elongation and side
    branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence
    on the plasma membrane of vegetative and generative cells of C. braunii. However,
    functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin
    transport, whereas CbPINc does not. The IAA is effective in the acceleration of
    cytoplasmic streaming and the phosphorylation of evolutionary conserved targets
    such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical
    PIN-mediated auxin transport mechanisms might not be fully conserved in Chara,
    IAA is involved in morphogenesis and fast signaling processes."
acknowledgement: 'This work was supported by funding from the Czech Science Foundation
  project no. 20-13587S to JP and SV, Charles University Grant Agency projects no.
  289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences
  to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge
  the Imaging Facility of the Institute of Experimental Botany AS CR supported by
  the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB
  AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large
  RI project LM2023050. Computational resources were provided by the e-INFRA CZ project
  (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner
  and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna
  Retzer and Jan Martinek for help with western blots, Anna Kampová for help with
  phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii
  strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated
  by Univerzita Karlova, as part of the Wiley - CzechELib agreement.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: K
  full_name: Kurtović, K
  last_name: Kurtović
- first_name: S
  full_name: Vosolsobě, S
  last_name: Vosolsobě
- first_name: D
  full_name: Nedvěd, D
  last_name: Nedvěd
- first_name: K
  full_name: Müller, K
  last_name: Müller
- first_name: PI
  full_name: Dobrev, PI
  last_name: Dobrev
- first_name: V
  full_name: Schmidt, V
  last_name: Schmidt
- first_name: P
  full_name: Piszczek, P
  last_name: Piszczek
- first_name: A
  full_name: Kuhn, A
  last_name: Kuhn
- first_name: Adrijana
  full_name: Smoljan, Adrijana
  id: cced8a85-223e-11ed-af04-b0596c55053b
  last_name: Smoljan
- first_name: TJ
  full_name: Fisher, TJ
  last_name: Fisher
- first_name: D
  full_name: Weijers, D
  last_name: Weijers
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: JL
  full_name: Bowman, JL
  last_name: Bowman
- first_name: J
  full_name: Petrášek, J
  last_name: Petrášek
citation:
  ama: Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid
    and characterization of PIN transporters in complex streptophyte alga Chara braunii.
    <i>New Phytologist</i>. 2025;246(3):1066-1083. doi:<a href="https://doi.org/10.1111/nph.70019">10.1111/nph.70019</a>
  apa: Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V.,
    … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of
    PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>.
    Wiley. <a href="https://doi.org/10.1111/nph.70019">https://doi.org/10.1111/nph.70019</a>
  chicago: Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek,
    et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters
    in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>. Wiley, 2025.
    <a href="https://doi.org/10.1111/nph.70019">https://doi.org/10.1111/nph.70019</a>.
  ieee: K. Kurtović <i>et al.</i>, “The role of indole-3-acetic acid and characterization
    of PIN transporters in complex streptophyte alga Chara braunii,” <i>New Phytologist</i>,
    vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.
  ista: Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek
    P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025.
    The role of indole-3-acetic acid and characterization of PIN transporters in complex
    streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.
  mla: Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization
    of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>,
    vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:<a href="https://doi.org/10.1111/nph.70019">10.1111/nph.70019</a>.
  short: K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P.
    Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J.
    Petrášek, New Phytologist 246 (2025) 1066–1083.
date_created: 2025-03-19T09:45:11Z
date_published: 2025-05-01T00:00:00Z
date_updated: 2025-09-30T11:11:18Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.70019
external_id:
  isi:
  - '001438711600001'
  pmid:
  - '40047465'
file:
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  date_created: 2025-04-16T08:03:36Z
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has_accepted_license: '1'
intvolume: '       246'
isi: 1
issue: '3'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1066-1083
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
  grant_number: I06123
  name: Peptide receptors for auxin canalization in Arabidopsis
publication: New Phytologist
publication_identifier:
  issn:
  - 1469-8137
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: The role of indole-3-acetic acid and characterization of PIN transporters in
  complex streptophyte alga Chara braunii
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 246
year: '2025'
...