{"ddc":["580"],"oa_version":"Published Version","author":[{"full_name":"Zhang, Jun","first_name":"Jun","last_name":"Zhang"},{"first_name":"Di","last_name":"Wu","full_name":"Wu, Di"},{"last_name":"Zhang","first_name":"Yating","full_name":"Zhang, Yating"},{"last_name":"Feng","first_name":"Xiaoqi","orcid":"0000-0002-4008-1234","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","full_name":"Feng, Xiaoqi"},{"id":"77c2e73a-eabd-11ef-aee9-8093a2ba7a93","full_name":"Gao, Hongbo","last_name":"Gao","first_name":"Hongbo"}],"OA_place":"publisher","_id":"19436","intvolume":"         5","type":"journal_article","date_published":"2025-03-04T00:00:00Z","scopus_import":"1","month":"03","year":"2025","article_processing_charge":"Yes","publication_identifier":{"eissn":["2730-9401"]},"DOAJ_listed":"1","file_date_updated":"2025-03-25T12:15:32Z","language":[{"iso":"eng"}],"date_created":"2025-03-23T23:01:25Z","article_number":"16","department":[{"_id":"XiFe"}],"oa":1,"doi":"10.1186/s43897-024-00137-9","acknowledgement":"We thank Prof. Ying Li of Nanjing Agricultural University for her help in providing seeds of K2 materials. This work was carried out with the support of National Natural Science Foundation of China (Grant No. 32070608).","abstract":[{"lang":"eng","text":"Dynamic DNA methylation represses transposable elements (TEs) and regulates gene activity, playing a pivotal role in plant development. Although substantial progress has been made in understanding DNA methylation reprogramming during germline development in Arabidopsis thaliana, whether similar mechanisms exist in other dicot plants remains unclear. Here, we analyzed DNA methylation levels in meiocytes, microspores, and pollens of Brassica Rapa using whole-genome bisulfite sequencing (WGBS). Global DNA methylation analysis revealed similar CHH methylation reprogramming compared to Arabidopsis, while distinct patterns were observed in the dynamics of global CG and CHG methylation in B. rapa. Differentially methylated region (DMR) analysis identified specifically methylated loci in the male sex cells of B. Rapa with a stronger tendency to target genes, similar to observations in Arabidopsis. Additionally, we found that the activity and genomic targeting preference of the small RNA-directed DNA methylation (RdDM) were altered during B. Rapa male germline development. A subset of long terminal repeat (LTR) TEs were activated, possibly due to the dynamic regulation of DNA methylation during male sexual development in B. Rapa. These findings provided new insights into the evolution of epigenetic reprogramming mechanisms in plants."}],"corr_author":"1","volume":5,"citation":{"ama":"Zhang J, Wu D, Zhang Y, Feng X, Gao H. DNA methylation dynamics in male germline development in Brassica Rapa. <i>Molecular Horticulture</i>. 2025;5. doi:<a href=\"https://doi.org/10.1186/s43897-024-00137-9\">10.1186/s43897-024-00137-9</a>","ista":"Zhang J, Wu D, Zhang Y, Feng X, Gao H. 2025. DNA methylation dynamics in male germline development in Brassica Rapa. Molecular Horticulture. 5, 16.","ieee":"J. Zhang, D. Wu, Y. Zhang, X. Feng, and H. Gao, “DNA methylation dynamics in male germline development in Brassica Rapa,” <i>Molecular Horticulture</i>, vol. 5. Springer Nature, 2025.","apa":"Zhang, J., Wu, D., Zhang, Y., Feng, X., &#38; Gao, H. (2025). DNA methylation dynamics in male germline development in Brassica Rapa. <i>Molecular Horticulture</i>. Springer Nature. <a href=\"https://doi.org/10.1186/s43897-024-00137-9\">https://doi.org/10.1186/s43897-024-00137-9</a>","short":"J. Zhang, D. Wu, Y. Zhang, X. Feng, H. Gao, Molecular Horticulture 5 (2025).","mla":"Zhang, Jun, et al. “DNA Methylation Dynamics in Male Germline Development in Brassica Rapa.” <i>Molecular Horticulture</i>, vol. 5, 16, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1186/s43897-024-00137-9\">10.1186/s43897-024-00137-9</a>.","chicago":"Zhang, Jun, Di Wu, Yating Zhang, Xiaoqi Feng, and Hongbo Gao. “DNA Methylation Dynamics in Male Germline Development in Brassica Rapa.” <i>Molecular Horticulture</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1186/s43897-024-00137-9\">https://doi.org/10.1186/s43897-024-00137-9</a>."},"publication":"Molecular Horticulture","publisher":"Springer Nature","article_type":"original","file":[{"file_id":"19460","creator":"dernst","success":1,"file_name":"2025_MolecularHorticulture_Zhang.pdf","date_updated":"2025-03-25T12:15:32Z","date_created":"2025-03-25T12:15:32Z","checksum":"6d1e0e9b0e1902e4a711f81c5c17a070","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_size":3014980}],"has_accepted_license":"1","quality_controlled":"1","date_updated":"2025-03-25T12:16:36Z","title":"DNA methylation dynamics in male germline development in Brassica Rapa","day":"04","status":"public","OA_type":"gold","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}}