---
res:
  bibo_abstract:
  - "Purpose: Optic nerve crush (ONC) is a model for studying optic nerve trauma.
    Unilateral ONC induces massive retinal ganglion cell (RGC) degeneration in the
    affected eye, leading to vision loss within a month. A common assumption has been
    that the non-injured contralateral eye is unaffected due to the minimal retino-retinal
    projections of the RGCs at the chiasm. Yet, recently, microglia, the brain-resident
    macrophages, have shown a responsive phenotype in the contralateral eye after
    ONC. Whether RGC loss accompanies this phenotype is still controversial.\r\n\r\nMethods:
    Using the available RGCode algorithm and developing our own RGC-Quant deep-learning-based
    tool, we quantify RGC's total number and density across the entire retina after
    ONC.\r\n\r\nResults: We confirm a short-term microglia response in the contralateral
    eye after ONC, but this did not affect the microglia number. Furthermore, we cannot
    confirm the previously reported RGC loss between naïve and contralateral retinas
    5 weeks after ONC induction across the commonly used Cx3cr1creERT2 and C57BL6/J
    mouse models. Neither sex nor the direct comparison of the RGC markers Brn3a and
    RBPMS, with Brn3a co-labeling, on average, 89% of the RBPMS+-cells, explained
    this discrepancy, suggesting that the early microglia-responsive phenotype does
    not have immediate consequences on the RGC number.\r\n\r\nConclusions: Our results
    corroborate that unilateral optic nerve injury elicits a microglial response in
    the uninjured contralateral eye but without RGC loss. Therefore, the contralateral
    eye should be treated separately and not as an ONC control.@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Florianne E
      foaf_name: Schoot Uiterkamp, Florianne E
      foaf_surname: Schoot Uiterkamp
      foaf_workInfoHomepage: http://www.librecat.org/personId=3526230C-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Margaret E
      foaf_name: Maes, Margaret E
      foaf_surname: Maes
      foaf_workInfoHomepage: http://www.librecat.org/personId=3838F452-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9642-1085
  - foaf_Person:
      foaf_givenName: Mohammad
      foaf_name: Alamalhoda, Mohammad
      foaf_surname: Alamalhoda
  - foaf_Person:
      foaf_givenName: Arsalan
      foaf_name: Firoozi, Arsalan
      foaf_surname: Firoozi
  - foaf_Person:
      foaf_givenName: Gloria
      foaf_name: Colombo, Gloria
      foaf_surname: Colombo
      foaf_workInfoHomepage: http://www.librecat.org/personId=3483CF6C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9434-8902
  - foaf_Person:
      foaf_givenName: Sandra
      foaf_name: Siegert, Sandra
      foaf_surname: Siegert
      foaf_workInfoHomepage: http://www.librecat.org/personId=36ACD32E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8635-0877
  bibo_doi: 10.1167/iovs.66.3.49
  bibo_issue: '3'
  bibo_volume: 66
  dct_date: 2025^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1552-5783
  dct_language: eng
  dct_publisher: Association for Research in Vision and Ophthalmology@
  dct_title: Optic nerve crush does not induce retinal ganglion cell loss in the contralateral
    eye@
  fabio_hasPubmedId: '40126507'
...