---
res:
  bibo_abstract:
  - N4-methylcytosine (4mC) is an important DNA modification in prokaryotes, but its
    relevance and even its presence in eukaryotes have been mysterious. Here we show
    that spermatogenesis in the liverwort Marchantia polymorpha involves two waves
    of extensive DNA methylation reprogramming. First, 5-methylcytosine (5mC) expands
    from transposons to the entire genome. Notably, the second wave installs 4mC throughout
    genic regions, covering over 50% of CG sites in sperm. 4mC requires a methyltransferase
    (MpDN4MT1a) that is specifically expressed during late spermiogenesis. Deletion
    of MpDN4MT1a alters the sperm transcriptome, causes sperm swimming and fertility
    defects, and impairs post-fertilization development. Our results reveal extensive
    4mC in a eukaryote, identify a family of eukaryotic methyltransferases, and elucidate
    the biological functions of 4mC in reproductive development, thereby expanding
    the repertoire of functional eukaryotic DNA modifications.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: James
      foaf_name: Walker, James
      foaf_surname: Walker
  - foaf_Person:
      foaf_givenName: Jingyi
      foaf_name: Zhang, Jingyi
      foaf_surname: Zhang
  - foaf_Person:
      foaf_givenName: Yalin
      foaf_name: Liu, Yalin
      foaf_surname: Liu
  - foaf_Person:
      foaf_givenName: Shujuan
      foaf_name: Xu, Shujuan
      foaf_surname: Xu
      foaf_workInfoHomepage: http://www.librecat.org/personId=9724dd9d-f591-11ee-bd51-e97ed0652286
  - foaf_Person:
      foaf_givenName: Yiming
      foaf_name: Yu, Yiming
      foaf_surname: Yu
      foaf_workInfoHomepage: http://www.librecat.org/personId=318e643b-8b61-11ed-b69e-aafa103ec8dd
    orcid: 0000-0002-9919-7282
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Vickers, Martin
      foaf_surname: Vickers
  - foaf_Person:
      foaf_givenName: Weizhi
      foaf_name: Ouyang, Weizhi
      foaf_surname: Ouyang
      foaf_workInfoHomepage: http://www.librecat.org/personId=fec73395-8b60-11ed-b69e-927fda99c743
  - foaf_Person:
      foaf_givenName: Judit
      foaf_name: Tálas, Judit
      foaf_surname: Tálas
  - foaf_Person:
      foaf_givenName: Liam
      foaf_name: Dolan, Liam
      foaf_surname: Dolan
  - foaf_Person:
      foaf_givenName: Keiji
      foaf_name: Nakajima, Keiji
      foaf_surname: Nakajima
  - foaf_Person:
      foaf_givenName: Xiaoqi
      foaf_name: Feng, Xiaoqi
      foaf_surname: Feng
      foaf_workInfoHomepage: http://www.librecat.org/personId=e0164712-22ee-11ed-b12a-d80fcdf35958
    orcid: 0000-0002-4008-1234
  bibo_doi: 10.1016/j.cell.2025.03.014
  bibo_issue: '11'
  bibo_volume: 188
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001504744800006
  dct_isPartOf:
  - http://id.crossref.org/issn/0092-8674
  - http://id.crossref.org/issn/1097-4172
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Extensive N4 cytosine methylation is essential for Marchantia sperm function@
  fabio_hasPubmedId: '40209706'
...