@unpublished{19717,
  abstract     = {Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the cerebral cortex through incompletely understood processes. Herein, we combine Mosaic Analysis with Double Markers (MADM)-based clonal analysis at embryonic days 12.5 and 13.5 with early postnatal callosal tracing to reveal a lineage progression that challenges the inside-outside model of cortical development and the conventional view of an invariable sequence of asymmetric neurogenic divisions. Our data demonstrate that early multipotent RGPs generate all extra-telencephalic (ET) and intra-telencephalic (IT) PNs across all layers through parallel sublineages and the random specification, during the earliest neurogenic divisions, of fate-restricted daughter RGPs. While the neuronal production of the parental multipotent RGPs consists of small ET-PN or IT-PN outputs, fate-restricted RGPs produce larger translaminar outputs spanning deep and upper layers of only IT-PNs, the predominant mammalian PN subtype. We further show that the emergence of IT-PN fate-restricted RGPs also leads to quantitatively and temporally stereotyped neurogenesis population-wise.},
  author       = {Varela-Martínez, I and Villalba Requena, Ana and Garcia-Marqués, J. and Hippenmeyer, Simon and Nieto, M.},
  booktitle    = {bioRxiv},
  title        = {{Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis}},
  doi          = {10.1101/2025.05.07.652665},
  year         = {2025},
}