---
res:
  bibo_abstract:
  - Protein-protein interactions (PPIs) mediate many fundamental cellular processes.
    Control of PPIs through optically or chemically responsive protein domains has
    had a profound impact on basic research and some clinical applications. Most chemogenetic
    methods induce the association, i.e., dimerization or oligomerization, of target
    proteins, whilst the few available dissociation approaches either break large
    oligomeric protein clusters or heteromeric complexes. Here, we have exploited
    the controlled dissociation of a homodimeric oxidoreductase from mycobacteria
    (MSMEG_2027) by its native cofactor, F420, which is not present in mammals, as
    a bioorthogonal monomerization switch. Using X-ray crystallography, we found that
    in the absence of F420 MSMEG_2027 forms a unique domain-swapped dimer that occludes
    the cofactor binding site. Rearrangement of the N-terminal helix upon F420 binding
    results in the dissolution of the dimer. We then showed that MSMEG_2027 can be
    fused to proteins of interest in human cells and applied it as a tool to induce
    and release MAPK/ERK signalling downstream of a chimeric fibroblast growth factor
    receptor 1 (FGFR1) tyrosine kinase. This F420-dependent chemogenetic de-homodimerization
    tool is stoichiometric and based on a single domain and thus represents a novel
    mechanism to investigate protein complexes in situ.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: James
      foaf_name: Antoney, James
      foaf_surname: Antoney
  - foaf_Person:
      foaf_givenName: Stephanie
      foaf_name: Kainrath, Stephanie
      foaf_surname: Kainrath
      foaf_workInfoHomepage: http://www.librecat.org/personId=32CFBA64-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-6709-2195
  - foaf_Person:
      foaf_givenName: Joshua G.
      foaf_name: Dubowsky, Joshua G.
      foaf_surname: Dubowsky
  - foaf_Person:
      foaf_givenName: F. Hafna
      foaf_name: Ahmed, F. Hafna
      foaf_surname: Ahmed
  - foaf_Person:
      foaf_givenName: Suk Woo
      foaf_name: Kang, Suk Woo
      foaf_surname: Kang
  - foaf_Person:
      foaf_givenName: Emily R.R.
      foaf_name: Mackie, Emily R.R.
      foaf_surname: Mackie
  - foaf_Person:
      foaf_givenName: Gustavo
      foaf_name: Bracho Granado, Gustavo
      foaf_surname: Bracho Granado
  - foaf_Person:
      foaf_givenName: Tatiana P.
      foaf_name: Soares Da Costa, Tatiana P.
      foaf_surname: Soares Da Costa
  - foaf_Person:
      foaf_givenName: Colin J.
      foaf_name: Jackson, Colin J.
      foaf_surname: Jackson
  - foaf_Person:
      foaf_givenName: Harald L
      foaf_name: Janovjak, Harald L
      foaf_surname: Janovjak
      foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8023-9315
  bibo_doi: 10.1016/j.jmb.2025.169184
  bibo_issue: '17'
  bibo_volume: 437
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001494762800001
  dct_isPartOf:
  - http://id.crossref.org/issn/0022-2836
  - http://id.crossref.org/issn/1089-8638
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: A F420-dependent single domain chemogenetic tool for protein de-dimerization@
  fabio_hasPubmedId: '40324743'
...