article published yes Vincent K Bett author 57854184-AAE0-11E9-8D04-98D6E5697425 Minerva S Trejo Arellano author 2b681148-eed5-11eb-b81b-ae229e8620f80000-0002-1982-3475 Beatriz Vicoso author 49E1C5C6-F248-11E8-B48F-1D18A9856A870000-0002-4579-8306 BeVi department DaZi department Sex chromosomes in evolution and development project The highjacking of meiosis for asexual reproduction project The males and females of the brine shrimp Artemia franciscana are highly dimorphic, and this dimorphism is associated with substantial sex-biased gene expression in heads and gonads. How these sex-specific patterns of expression are regulated at the molecular level is unknown. A. franciscana also has differentiated ZW sex chromosomes, with complete dosage compensation, but the molecular mechanism through which compensation is achieved is unknown. Here, we conducted CUT&TAG assays targeting 7 post-translational histone modifications (H3K27me3, H3K9me2, H3K9me3, H3K36me3, H3K27ac, H3K4me3, and H4K16ac) in heads and gonads of A. franciscana, allowing us to divide the genome into 12 chromatin states. We further defined functional chromatin signatures for all genes, which were correlated with transcript level abundances. Differences in the occupancy of the profiled epigenetic marks between sexes were associated with differential gene expression between males and females. Finally, we found a significant enrichment of the permissive H4K16ac histone mark in the Z-specific region in both tissues of females but not males, supporting the role of this histone mark in mediating dosage compensation of the Z chromosome. https://research-explorer.ista.ac.at/download/19735/19756/2025_MBE_Bett.pdf application/pdfno Oxford University Press2025 eng 0737-4038 1537-1719 40202086 00148346020000110.1093/molbev/msaf085 425 https://research-explorer.ista.ac.at/record/20444 https://research-explorer.ista.ac.at/record/20449 https://github.com/vkb25/Chromatin-landscape-in-Artemia-franciscana.git Bett, Vincent K., et al. “Chromatin Landscape Is Associated with Sex-Biased Expression and Drosophila-like Dosage Compensation of the Z Chromosome in Artemia Franciscana.” <i>Molecular Biology and Evolution</i>, vol. 42, no. 5, msaf085, Oxford University Press, 2025, doi:<a href="https://doi.org/10.1093/molbev/msaf085">10.1093/molbev/msaf085</a>. Bett VK, Trejo Arellano MS, Vicoso B. Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. <i>Molecular Biology and Evolution</i>. 2025;42(5). doi:<a href="https://doi.org/10.1093/molbev/msaf085">10.1093/molbev/msaf085</a> Bett, Vincent K, Minerva S Trejo Arellano, and Beatriz Vicoso. “Chromatin Landscape Is Associated with Sex-Biased Expression and Drosophila-like Dosage Compensation of the Z Chromosome in Artemia Franciscana.” <i>Molecular Biology and Evolution</i>. Oxford University Press, 2025. <a href="https://doi.org/10.1093/molbev/msaf085">https://doi.org/10.1093/molbev/msaf085</a>. Bett VK, Trejo Arellano MS, Vicoso B. 2025. Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. Molecular Biology and Evolution. 42(5), msaf085. V. K. Bett, M. S. Trejo Arellano, and B. Vicoso, “Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana,” <i>Molecular Biology and Evolution</i>, vol. 42, no. 5. Oxford University Press, 2025. Bett, V. K., Trejo Arellano, M. S., &#38; Vicoso, B. (2025). Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. <i>Molecular Biology and Evolution</i>. Oxford University Press. <a href="https://doi.org/10.1093/molbev/msaf085">https://doi.org/10.1093/molbev/msaf085</a> V.K. Bett, M.S. Trejo Arellano, B. Vicoso, Molecular Biology and Evolution 42 (2025). 197352025-05-25T22:16:56Z2026-04-07T12:28:15Z