[{"abstract":[{"lang":"eng"}],"acknowledgement":"This work was supported by the Medical Research Council. ","quality_controlled":0,"month":"02","intvolume":" 286","publication_status":"published","day":"18","publication":"Journal of Biological Chemistry","dc":{"publisher":["American Society for Biochemistry and Molecular Biology"],"type":["info:eu-repo/semantics/article","doc-type:article","text","http://purl.org/coar/resource_type/c_6501"],"date":["2011"],"description":["Modern α-proteobacteria are thought to be closely related to the ancient symbiont of eukaryotes, an ancestor of mitochondria. Respiratory complex I from α-proteobacteria and mitochondria is well conserved at the level of the 14 "core" subunits, consistent with that notion. Mitochondrial complex I contains the core subunits, present in all species, and up to 31 "supernumerary" subunits, generally thought to have originated only within eukaryotic lineages. However, the full protein composition of an α-proteobacterial complex I has not been established previously. Here, we report the first purification and characterization of complex I from the α-proteobacterium Paracoccus denitrificans. Single particle electron microscopy shows that the complex has a well defined L-shape. Unexpectedly, in addition to the 14 core subunits, the enzyme also contains homologues of three supernumerary mitochondrial subunits as follows: B17.2, AQDQ/18, and 13 kDa (bovine nomenclature). This finding suggests that evolution of complex I via addition of supernumerary or "accessory" subunits started before the original endosymbiotic event that led to the creation of the eukaryotic cell. It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria."],"identifier":["https://research-explorer.ista.ac.at/record/1975"],"relation":["info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M110.194993"],"source":["Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 2011;286(7):5023-5033. doi:10.1074/jbc.M110.194993"],"title":["Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme"],"creator":["Yip, Chui Y","Harbour, Michael E","Jayawardena, Kamburapola G","Fearnley, Ian M","Leonid Sazanov"],"rights":["info:eu-repo/semantics/closedAccess"]},"page":"5023 - 5033","uri_base":"https://research-explorer.ista.ac.at","issue":"7","volume":286,"date_published":"2011-02-18T00:00:00Z","date_created":"2018-12-11T11:55:00Z","_id":"1975","type":"journal_article","status":"public","citation":{"chicago":"Yip, Chui, Michael Harbour, Kamburapola Jayawardena, Ian Fearnley, and Leonid A Sazanov. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2011. https://doi.org/10.1074/jbc.M110.194993.","ista":"Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. 2011. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 286(7), 5023–5033.","mla":"Yip, Chui, et al. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry, vol. 286, no. 7, American Society for Biochemistry and Molecular Biology, 2011, pp. 5023–33, doi:10.1074/jbc.M110.194993.","apa":"Yip, C., Harbour, M., Jayawardena, K., Fearnley, I., & Sazanov, L. A. (2011). Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M110.194993","short":"C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, L.A. Sazanov, Journal of Biological Chemistry 286 (2011) 5023–5033.","ieee":"C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, and L. A. Sazanov, “Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme,” Journal of Biological Chemistry, vol. 286, no. 7. American Society for Biochemistry and Molecular Biology, pp. 5023–5033, 2011."},"dini_type":"doc-type:article","date_updated":"2021-01-12T06:54:27Z","extern":1,"author":[{"first_name":"Chui","last_name":"Yip"},{"first_name":"Michael","last_name":"Harbour"},{"first_name":"Kamburapola","last_name":"Jayawardena"},{"first_name":"Ian","last_name":"Fearnley"},{"id":"338D39FE-F248-11E8-B48F-1D18A9856A87","first_name":"Leonid A","orcid":"0000-0002-0977-7989","last_name":"Sazanov"}],"publist_id":"5112"}]