---
_id: '1984'
abstract:
- lang: eng
  text: In animal and plant cells, mitotic chromatin locally generates microtubules
    that self-organize into a mitotic spindle, and its dimensions and bipolar symmetry
    are essential for accurate chromosome segregation. By immobilizing microscopic
    chromatin-coated beads on slide surfaces using a microprinting technique, we have
    examined the effect of chromatin on the dimensions and symmetry of spindles in
    Xenopus laevis cytoplasmic extracts. While circular spots with diameters around
    14-18 μm trigger bipolar spindle formation, larger spots generate an incorrect
    number of poles. We also examined lines of chromatin with various dimensions.
    Their length determined the number of poles that formed, with a 6 × 18 μm rectangular
    patch generating normal spindle morphology. Around longer lines, multiple poles
    formed and the structures were disorganized. While lines thinner than 10 μm generated
    symmetric structures, thicker lines induced the formation of asymmetric structures
    where all microtubules are on the same side of the line. Our results show that
    chromatin defines spindle shape and orientation. For a video summary of this article,
    see the PaperFlick file available with the online Supplemental Data.
acknowledgement: This work was supported by EU contract LSHG-CT-2004-503568 ComBio,
  the Spanish ministry of education (M.M.C.), and EU-STREP active BioMics (A.D.).
  Research in the Nedelec lab is funded by the Center for Modeling and Simulation
  in the Biosciences (http://www.bioms.de), the Volkswagenstiftung, and Human Frontier
  Science Program grant RGY84.
author:
- first_name: Ana
  full_name: Dinarina, Ana
  last_name: Dinarina
- first_name: Céline
  full_name: Pugieux, Céline
  last_name: Pugieux
- first_name: Maria
  full_name: Corral, Maria M
  last_name: Corral
- first_name: Martin
  full_name: Martin Loose
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
- first_name: Joachim
  full_name: Spatz, Joachim P
  last_name: Spatz
- first_name: Éric
  full_name: Karsenti, Éric
  last_name: Karsenti
- first_name: François
  full_name: Nédélec, François J
  last_name: Nédélec
citation:
  ama: Dinarina A, Pugieux C, Corral M, et al. Chromatin shapes the mitotic spindle.
    <i>Cell</i>. 2009;138(3):502-513. doi:<a href="https://doi.org/10.1016/j.cell.2009.05.027">10.1016/j.cell.2009.05.027</a>
  apa: Dinarina, A., Pugieux, C., Corral, M., Loose, M., Spatz, J., Karsenti, É.,
    &#38; Nédélec, F. (2009). Chromatin shapes the mitotic spindle. <i>Cell</i>. Cell
    Press. <a href="https://doi.org/10.1016/j.cell.2009.05.027">https://doi.org/10.1016/j.cell.2009.05.027</a>
  chicago: Dinarina, Ana, Céline Pugieux, Maria Corral, Martin Loose, Joachim Spatz,
    Éric Karsenti, and François Nédélec. “Chromatin Shapes the Mitotic Spindle.” <i>Cell</i>.
    Cell Press, 2009. <a href="https://doi.org/10.1016/j.cell.2009.05.027">https://doi.org/10.1016/j.cell.2009.05.027</a>.
  ieee: A. Dinarina <i>et al.</i>, “Chromatin shapes the mitotic spindle,” <i>Cell</i>,
    vol. 138, no. 3. Cell Press, pp. 502–513, 2009.
  ista: Dinarina A, Pugieux C, Corral M, Loose M, Spatz J, Karsenti É, Nédélec F.
    2009. Chromatin shapes the mitotic spindle. Cell. 138(3), 502–513.
  mla: Dinarina, Ana, et al. “Chromatin Shapes the Mitotic Spindle.” <i>Cell</i>,
    vol. 138, no. 3, Cell Press, 2009, pp. 502–13, doi:<a href="https://doi.org/10.1016/j.cell.2009.05.027">10.1016/j.cell.2009.05.027</a>.
  short: A. Dinarina, C. Pugieux, M. Corral, M. Loose, J. Spatz, É. Karsenti, F. Nédélec,
    Cell 138 (2009) 502–513.
date_created: 2018-12-11T11:55:03Z
date_published: 2009-08-07T00:00:00Z
date_updated: 2021-01-12T06:54:30Z
day: '07'
doi: 10.1016/j.cell.2009.05.027
extern: 1
intvolume: '       138'
issue: '3'
month: '08'
page: 502 - 513
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5100'
quality_controlled: 0
status: public
title: Chromatin shapes the mitotic spindle
type: journal_article
volume: 138
year: '2009'
...