---
res:
  bibo_abstract:
  - The Ca2+-release-activated Ca2+ (CRAC) channel Orai1 is activated by interaction
    with the Ca2+ sensor Stromal Interaction Molecule 1 (STIM1). Owing to the lack
    of structurally resolved Orai1/STIM1 complexes, the impact of their coupling on
    individual Orai1 transmembrane domain (TM) movements is unclear. This study investigates
    STIM1-independent and STIM1-dependent Orai1-TM dynamics using photocrosslinking
    unnatural amino acids (UAAs) at each individual TM position. We primarily identify
    CRAC-channel-like currents directly after UAA incorporation or additional UV-light
    irradiation at TM3 sites that interface with non-pore-lining TMs. Using UAAs combined
    with conventional site-directed mutagenesis and molecular dynamics simulations,
    we discover that pore opening involves a widening of interfaces formed by TM3
    with non-pore-lining TMs. Orai1 mutants with a UAA in TM3 exhibit weaker STIM1-induced
    activation after UV exposure, possibly caused by a restricted widening of non-pore-lining
    TM interfaces. We demonstrate that photocrosslinking UAAs are excellent tools
    for improving our understanding of key determinants and ion channel dynamics modulating
    pore opening.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Hadil
      foaf_name: Najjar, Hadil
      foaf_surname: Najjar
  - foaf_Person:
      foaf_givenName: Sarah
      foaf_name: Weiß, Sarah
      foaf_surname: Weiß
  - foaf_Person:
      foaf_givenName: Ferdinand
      foaf_name: Horvath, Ferdinand
      foaf_surname: Horvath
      foaf_workInfoHomepage: http://www.librecat.org/personId=b0dc7f61-21a3-11ef-a9b4-e6ab1aa6f21e
  - foaf_Person:
      foaf_givenName: Valentina
      foaf_name: Hopl, Valentina
      foaf_surname: Hopl
  - foaf_Person:
      foaf_givenName: Adéla
      foaf_name: Tiffner, Adéla
      foaf_surname: Tiffner
  - foaf_Person:
      foaf_givenName: Lorenz
      foaf_name: Höbarth, Lorenz
      foaf_surname: Höbarth
  - foaf_Person:
      foaf_givenName: Julia
      foaf_name: Söllner, Julia
      foaf_surname: Söllner
  - foaf_Person:
      foaf_givenName: Maximilian
      foaf_name: Fröhlich, Maximilian
      foaf_surname: Fröhlich
  - foaf_Person:
      foaf_givenName: Magdalena
      foaf_name: Prantl, Magdalena
      foaf_surname: Prantl
  - foaf_Person:
      foaf_givenName: Nora
      foaf_name: Müller, Nora
      foaf_surname: Müller
  - foaf_Person:
      foaf_givenName: Yuliia
      foaf_name: Nazarenko, Yuliia
      foaf_surname: Nazarenko
  - foaf_Person:
      foaf_givenName: Selina
      foaf_name: Harant, Selina
      foaf_surname: Harant
  - foaf_Person:
      foaf_givenName: Lukas
      foaf_name: Weissenböck, Lukas
      foaf_surname: Weissenböck
  - foaf_Person:
      foaf_givenName: Herwig
      foaf_name: Grabmayr, Herwig
      foaf_surname: Grabmayr
  - foaf_Person:
      foaf_givenName: Matthias
      foaf_name: Sallinger, Matthias
      foaf_surname: Sallinger
  - foaf_Person:
      foaf_givenName: Lena
      foaf_name: Maltan, Lena
      foaf_surname: Maltan
  - foaf_Person:
      foaf_givenName: Linda V.
      foaf_name: Echefu, Linda V.
      foaf_surname: Echefu
  - foaf_Person:
      foaf_givenName: Tamara
      foaf_name: Radiskovic, Tamara
      foaf_surname: Radiskovic
  - foaf_Person:
      foaf_givenName: Melanie
      foaf_name: Leopold, Melanie
      foaf_surname: Leopold
  - foaf_Person:
      foaf_givenName: Sonja
      foaf_name: Lindinger, Sonja
      foaf_surname: Lindinger
  - foaf_Person:
      foaf_givenName: Christina
      foaf_name: Humer, Christina
      foaf_surname: Humer
  - foaf_Person:
      foaf_givenName: Carmen
      foaf_name: Höglinger, Carmen
      foaf_surname: Höglinger
  - foaf_Person:
      foaf_givenName: Heinrich
      foaf_name: Krobath, Heinrich
      foaf_surname: Krobath
  - foaf_Person:
      foaf_givenName: Thomas
      foaf_name: Renger, Thomas
      foaf_surname: Renger
  - foaf_Person:
      foaf_givenName: Isabella
      foaf_name: Derler, Isabella
      foaf_surname: Derler
  bibo_doi: 10.1016/j.xcrp.2025.102623
  bibo_issue: '6'
  bibo_volume: 6
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001516570500009
  dct_isPartOf:
  - http://id.crossref.org/issn/2666-3864
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: STIM1-induced widening of non-pore-lining TM interfaces is crucial for
    Orai1 pore opening@
...