{"title":"Spatial organization of cytokinesis signaling reconstituted in a cell-free system","quality_controlled":0,"intvolume":"       346","date_published":"2014-10-10T00:00:00Z","extern":1,"citation":{"ista":"Nguyen P, Groen A, Loose M, Ishihara K, Wühr M, Field C, Mitchison T. 2014. Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Science. 346(6206), 244–247.","apa":"Nguyen, P., Groen, A., Loose, M., Ishihara, K., Wühr, M., Field, C., &#38; Mitchison, T. (2014). Spatial organization of cytokinesis signaling reconstituted in a cell-free system. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1256773\">https://doi.org/10.1126/science.1256773</a>","chicago":"Nguyen, Phuong, Aaron Groen, Martin Loose, Keisuke Ishihara, Martin Wühr, Christine Field, and Timothy Mitchison. “Spatial Organization of Cytokinesis Signaling Reconstituted in a Cell-Free System.” <i>Science</i>. American Association for the Advancement of Science, 2014. <a href=\"https://doi.org/10.1126/science.1256773\">https://doi.org/10.1126/science.1256773</a>.","ieee":"P. Nguyen <i>et al.</i>, “Spatial organization of cytokinesis signaling reconstituted in a cell-free system,” <i>Science</i>, vol. 346, no. 6206. American Association for the Advancement of Science, pp. 244–247, 2014.","ama":"Nguyen P, Groen A, Loose M, et al. Spatial organization of cytokinesis signaling reconstituted in a cell-free system. <i>Science</i>. 2014;346(6206):244-247. doi:<a href=\"https://doi.org/10.1126/science.1256773\">10.1126/science.1256773</a>","mla":"Nguyen, Phuong, et al. “Spatial Organization of Cytokinesis Signaling Reconstituted in a Cell-Free System.” <i>Science</i>, vol. 346, no. 6206, American Association for the Advancement of Science, 2014, pp. 244–47, doi:<a href=\"https://doi.org/10.1126/science.1256773\">10.1126/science.1256773</a>.","short":"P. Nguyen, A. Groen, M. Loose, K. Ishihara, M. Wühr, C. Field, T. Mitchison, Science 346 (2014) 244–247."},"month":"10","year":"2014","publisher":"American Association for the Advancement of Science","day":"10","status":"public","date_created":"2018-12-11T11:55:04Z","author":[{"full_name":"Nguyen, Phuong A","last_name":"Nguyen","first_name":"Phuong"},{"last_name":"Groen","first_name":"Aaron","full_name":"Groen, Aaron C"},{"full_name":"Martin Loose","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","orcid":"0000-0001-7309-9724","last_name":"Loose"},{"first_name":"Keisuke","last_name":"Ishihara","full_name":"Ishihara, Keisuke "},{"first_name":"Martin","last_name":"Wühr","full_name":"Wühr, Martin "},{"first_name":"Christine","last_name":"Field","full_name":"Field, Christine M"},{"last_name":"Mitchison","first_name":"Timothy","full_name":"Mitchison, Timothy J"}],"volume":346,"abstract":[{"text":"During animal cell division, the cleavage furrow is positioned by microtubules that signal to the actin cortex at the cell midplane. We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid bilayers to mimic the plasma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps. This system opens further approaches to understanding the biophysics of cytokinesis signaling.","lang":"eng"}],"date_updated":"2021-01-12T06:54:32Z","publist_id":"5093","_id":"1989","page":"244 - 247","doi":"10.1126/science.1256773","type":"journal_article","publication_status":"published","issue":"6206","acknowledgement":"This work was supported by NIH grant GM39565 (T.J.M.); MBL fellowships from the Evans Foundation, MBL Associates, and the Colwin Fund (T.J.M. and C.M.F.); HFSP fellowship LT000466/2012-L (M.L.); and NIH grant GM103785 (M.W.). ","publication":"Science"}