---
_id: '1989'
abstract:
- lang: eng
text: During animal cell division, the cleavage furrow is positioned by microtubules
that signal to the actin cortex at the cell midplane. We developed a cell-free
system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus
eggs. Microtubules grew out as asters from artificial centrosomes and met to organize
antiparallel overlap zones. These zones blocked the interpenetration of neighboring
asters and recruited cytokinesis midzone proteins, including the chromosomal passenger
complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which
required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid
bilayers to mimic the plasma membrane, we observed the recruitment of cleavage
furrow markers, including an active RhoA reporter, at microtubule overlaps. This
system opens further approaches to understanding the biophysics of cytokinesis
signaling.
acknowledgement: 'This work was supported by NIH grant GM39565 (T.J.M.); MBL fellowships
from the Evans Foundation, MBL Associates, and the Colwin Fund (T.J.M. and C.M.F.);
HFSP fellowship LT000466/2012-L (M.L.); and NIH grant GM103785 (M.W.). '
author:
- first_name: Phuong
full_name: Nguyen, Phuong A
last_name: Nguyen
- first_name: Aaron
full_name: Groen, Aaron C
last_name: Groen
- first_name: Martin
full_name: Martin Loose
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Keisuke
full_name: 'Ishihara, Keisuke '
last_name: Ishihara
- first_name: Martin
full_name: 'Wühr, Martin '
last_name: Wühr
- first_name: Christine
full_name: Field, Christine M
last_name: Field
- first_name: Timothy
full_name: Mitchison, Timothy J
last_name: Mitchison
citation:
ama: Nguyen P, Groen A, Loose M, et al. Spatial organization of cytokinesis signaling
reconstituted in a cell-free system. Science. 2014;346(6206):244-247. doi:10.1126/science.1256773
apa: Nguyen, P., Groen, A., Loose, M., Ishihara, K., Wühr, M., Field, C., &
Mitchison, T. (2014). Spatial organization of cytokinesis signaling reconstituted
in a cell-free system. Science. American Association for the Advancement
of Science. https://doi.org/10.1126/science.1256773
chicago: Nguyen, Phuong, Aaron Groen, Martin Loose, Keisuke Ishihara, Martin Wühr,
Christine Field, and Timothy Mitchison. “Spatial Organization of Cytokinesis Signaling
Reconstituted in a Cell-Free System.” Science. American Association for
the Advancement of Science, 2014. https://doi.org/10.1126/science.1256773.
ieee: P. Nguyen et al., “Spatial organization of cytokinesis signaling reconstituted
in a cell-free system,” Science, vol. 346, no. 6206. American Association
for the Advancement of Science, pp. 244–247, 2014.
ista: Nguyen P, Groen A, Loose M, Ishihara K, Wühr M, Field C, Mitchison T. 2014.
Spatial organization of cytokinesis signaling reconstituted in a cell-free system.
Science. 346(6206), 244–247.
mla: Nguyen, Phuong, et al. “Spatial Organization of Cytokinesis Signaling Reconstituted
in a Cell-Free System.” Science, vol. 346, no. 6206, American Association
for the Advancement of Science, 2014, pp. 244–47, doi:10.1126/science.1256773.
short: P. Nguyen, A. Groen, M. Loose, K. Ishihara, M. Wühr, C. Field, T. Mitchison,
Science 346 (2014) 244–247.
date_created: 2018-12-11T11:55:04Z
date_published: 2014-10-10T00:00:00Z
date_updated: 2021-01-12T06:54:32Z
day: '10'
doi: 10.1126/science.1256773
extern: 1
intvolume: ' 346'
issue: '6206'
month: '10'
page: 244 - 247
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5093'
quality_controlled: 0
status: public
title: Spatial organization of cytokinesis signaling reconstituted in a cell-free
system
type: journal_article
volume: 346
year: '2014'
...