---
_id: '19915'
acknowledgement: "We thank all members of the Martin Loose lab at ISTA for helpful
  discussions and Marko Kojic for critical reading of the manuscript. This research
  was supported by the Scientific Service Units (SSU) of ISTA through resources provided
  by the Imaging & Optics Facility (IOF), the Scientific Computing (SciComp) and the
  Electron Microscopy Facility (EMF), as well as the Lab Support Facility (LSF). This
  project has received funding from the European Union’s Horizon 2020 research and
  innovation program under the Marie Skłodowska-Curie Grant Agreement No.101034413
  awarded to BLS as well as an ERC grant (ActinID, 101076260) from the European Union
  awarded to FKMS. Views and opinions expressed are however those of the author(s)
  only and do not necessarily reflect those of the European Union or the European
  Research Council. Neither the European Union nor the granting authority can be held
  responsible for them.\r\n\r\nWe are grateful for Antonia Herrero (Sevilla University)
  for sharing her extensive BACTH plasmid library and other plasmids as well as cyanobacterial
  strains. Likewise, we would like to thank Tal Dagan and Fabian Nies (both Kiel University)
  for sharing cyanobacterial strains and plasmids and for valuable discussions.\r\n\r\nWe
  would further like to express our gratitude to Nicolas Sapay and Alexis Michon for
  providing the Amphipaseek code, which enabled us to perform our large-scale amphipathic
  helix screen of cyanobacterial CorR proteins. Finally, we also want to thank Jesse
  Hansen for advice in cryo-EM data processing"
article_processing_charge: No
author:
- first_name: Benjamin L
  full_name: Springstein, Benjamin L
  id: b4eb62ef-ac72-11ed-9503-ed3b4d66c083
  last_name: Springstein
  orcid: 0000-0002-3461-5391
citation:
  ama: Springstein BL. Files for “Evolutionary repurposing of a DNA segregation machinery
    into a cytoskeletal system controlling cyanobacterial cell shape.” 2025. doi:<a
    href="https://doi.org/10.15479/AT:ISTA:19915">10.15479/AT:ISTA:19915</a>
  apa: Springstein, B. L. (2025). Files for “Evolutionary repurposing of a DNA segregation
    machinery into a cytoskeletal system controlling cyanobacterial cell shape.” Institute
    of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:19915">https://doi.org/10.15479/AT:ISTA:19915</a>
  chicago: Springstein, Benjamin L. “Files for ‘Evolutionary Repurposing of a DNA
    Segregation Machinery into a Cytoskeletal System Controlling Cyanobacterial Cell
    Shape.’” Institute of Science and Technology Austria, 2025. <a href="https://doi.org/10.15479/AT:ISTA:19915">https://doi.org/10.15479/AT:ISTA:19915</a>.
  ieee: B. L. Springstein, “Files for ‘Evolutionary repurposing of a DNA segregation
    machinery into a cytoskeletal system controlling cyanobacterial cell shape.’”
    Institute of Science and Technology Austria, 2025.
  ista: Springstein BL. 2025. Files for ‘Evolutionary repurposing of a DNA segregation
    machinery into a cytoskeletal system controlling cyanobacterial cell shape’, Institute
    of Science and Technology Austria, <a href="https://doi.org/10.15479/AT:ISTA:19915">10.15479/AT:ISTA:19915</a>.
  mla: Springstein, Benjamin L. <i>Files for “Evolutionary Repurposing of a DNA Segregation
    Machinery into a Cytoskeletal System Controlling Cyanobacterial Cell Shape.”</i>
    Institute of Science and Technology Austria, 2025, doi:<a href="https://doi.org/10.15479/AT:ISTA:19915">10.15479/AT:ISTA:19915</a>.
  short: B.L. Springstein, (2025).
contributor:
- contributor_type: project_leader
  first_name: Benjamin L
  id: b4eb62ef-ac72-11ed-9503-ed3b4d66c083
  last_name: Springstein
  orcid: 0000-0002-3461-5391
- contributor_type: researcher
  first_name: Manjunath
  id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2
  last_name: Javoor
- contributor_type: researcher
  first_name: Daniela
  last_name: Megrian
- contributor_type: researcher
  first_name: Roman
  id: ffab949d-133f-11ed-8f02-94de21ace503
  last_name: Hajdu
- contributor_type: researcher
  first_name: Dustin M
  last_name: Hanke
- contributor_type: researcher
  first_name: Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
- contributor_type: supervisor
  first_name: Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
corr_author: '1'
date_created: 2025-06-27T07:34:52Z
date_published: 2025-06-27T00:00:00Z
date_updated: 2026-06-10T08:05:45Z
day: '27'
department:
- _id: MaLo
doi: 10.15479/AT:ISTA:19915
ec_funded: 1
file:
- access_level: open_access
  checksum: 5b85c299bdd46cbde000e3449da835e0
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19916'
  file_name: Supplementary Movie 1) in vivo time lapse microscopy of mNG-CorM.avi
  file_size: 2003892
  relation: main_file
  success: 1
- access_level: open_access
  checksum: a05c19dad5ac10bc05f349c3507a70a7
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:28Z
  date_updated: 2025-06-27T07:35:28Z
  file_id: '19917'
  file_name: Supplementary Movie 2) mNG-CorM single cell.avi
  file_size: 126618
  relation: main_file
  success: 1
- access_level: open_access
  checksum: b3ac037572b2c4cfed4212222915b268
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19918'
  file_name: Supplementary Movie 3) mNG-(1-40)CorM single cell.avi
  file_size: 383114
  relation: main_file
  success: 1
- access_level: open_access
  checksum: f4f6b3d1b6add03990f80edbfa14a688
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19919'
  file_name: Supplementary Movie 4) in vitro FRAP of CorM.avi
  file_size: 4188170
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 57e0cbcfdd347f75808c7ae049136f22
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19920'
  file_name: Supplementary Movie 5) in vitro FRAP of (1-40)CorM.avi
  file_size: 3862712
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 58d36666c1f3155d5a641e6b47f3843f
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19921'
  file_name: Supplementary Movie 6) in vitro CorM filament tracking.avi
  file_size: 3183046
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 509a0d7db16cf1db581b719d355718db
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19922'
  file_name: Supplementary Movie 7) in vitro CorM dual color imaging.avi
  file_size: 468600
  relation: main_file
  success: 1
- access_level: open_access
  checksum: e6e5f8755819503eeb7081049d3d6aa5
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19923'
  file_name: Supplementary Movie 8) in vivo MinC oscillation.avi
  file_size: 3038980
  relation: main_file
  success: 1
- access_level: open_access
  checksum: c6c0e4cd9766ecafce52d99a0ac8c339
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19924'
  file_name: Supplementary Movie 9) in vitro CorM + MinC.avi
  file_size: 21730482
  relation: main_file
  success: 1
- access_level: open_access
  checksum: abe15b253a7c9a1dc484a614dd2ab30a
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:29Z
  date_updated: 2025-06-27T07:35:29Z
  file_id: '19925'
  file_name: Supplementary Movie 10) in vitro CorM + MinC(S50A+L55D).avi
  file_size: 28315550
  relation: main_file
  success: 1
- access_level: open_access
  checksum: d5cc3426aa3e066ee175e6c3a66cf84d
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:30Z
  date_updated: 2025-06-27T07:35:30Z
  file_id: '19926'
  file_name: Supplementary Movie 11) in vitro CorM + MinC(delta46-67).avi
  file_size: 25693552
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 70daf423216753ec5c73afe18f347821
  content_type: video/x-msvideo
  creator: bsprings
  date_created: 2025-06-27T07:35:30Z
  date_updated: 2025-06-27T07:35:30Z
  file_id: '19927'
  file_name: Supplementary Movie 12) in vitro CorM + buffer ctrl.avi
  file_size: 25349196
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 215744428591d013c0193d6df842ae06
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: bsprings
  date_created: 2025-07-02T08:10:11Z
  date_updated: 2025-07-02T08:10:11Z
  file_id: '19944'
  file_name: Readme ISTA repository_Springstein 2025_v2.docx
  file_size: 46992
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 693e1c5a1fca4afcf428c325057636d7
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:21Z
  date_updated: 2025-07-02T08:10:21Z
  file_id: '19945'
  file_name: Main Figures.pdf
  file_size: 79915541
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 51ef5c8e98c388394746c90d04626443
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:19Z
  date_updated: 2025-07-02T08:10:19Z
  file_id: '19946'
  file_name: Supplementary Figure 1.pdf
  file_size: 6860499
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 18a416d158b8a74a199054ef618beb08
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:19Z
  date_updated: 2025-07-02T08:10:19Z
  file_id: '19947'
  file_name: Supplementary Figure 2.pdf
  file_size: 3025601
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 8d252711086b99123bd47a20a1bb96c5
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:19Z
  date_updated: 2025-07-02T08:10:19Z
  file_id: '19948'
  file_name: Supplementary Figure 3.pdf
  file_size: 2130970
  relation: main_file
  success: 1
- access_level: open_access
  checksum: c038000e7b5b1fed77888bcba8268e3a
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:19Z
  date_updated: 2025-07-02T08:10:19Z
  file_id: '19949'
  file_name: Supplementary Figure 4.pdf
  file_size: 583089
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 73e4ae52520ff1037336fd088139872f
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:19Z
  date_updated: 2025-07-02T08:10:19Z
  file_id: '19950'
  file_name: Supplementary Figure 5.pdf
  file_size: 81663
  relation: main_file
  success: 1
- access_level: open_access
  checksum: b5e75fda4fc9a2599972341777a8de4b
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:20Z
  date_updated: 2025-07-02T08:10:20Z
  file_id: '19951'
  file_name: Supplementary Figure 6.pdf
  file_size: 19661623
  relation: main_file
  success: 1
- access_level: open_access
  checksum: aab9ea12e09c12a97d33e3d2d44471e7
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:20Z
  date_updated: 2025-07-02T08:10:20Z
  file_id: '19952'
  file_name: Supplementary Figure 7.pdf
  file_size: 3756335
  relation: main_file
  success: 1
- access_level: open_access
  checksum: b4c7b6947a0aa2840b7dacc1489896df
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:20Z
  date_updated: 2025-07-02T08:10:20Z
  file_id: '19953'
  file_name: Supplementary Figure 8.pdf
  file_size: 3216820
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 8423814c4dfefad5757835aab8295b2e
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:21Z
  date_updated: 2025-07-02T08:10:21Z
  file_id: '19954'
  file_name: Supplementary Figure 9.pdf
  file_size: 7449121
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 31ecac7daf4b50e2ea443f398fdc2110
  content_type: application/pdf
  creator: bsprings
  date_created: 2025-07-02T08:10:21Z
  date_updated: 2025-07-02T08:10:21Z
  file_id: '19955'
  file_name: Supplementary Figure 10.pdf
  file_size: 6168864
  relation: main_file
  success: 1
file_date_updated: 2025-07-02T08:10:21Z
has_accepted_license: '1'
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
  call_identifier: H2020
  grant_number: '101034413'
  name: 'IST-BRIDGE: International postdoctoral program'
- _id: bd980d18-d553-11ed-ba76-ceaa645c97eb
  grant_number: '101076260'
  name: A molecular atlas of Actin filament IDentities in the cell motility machinery
publisher: Institute of Science and Technology Austria
status: public
title: Files for "Evolutionary repurposing of a DNA segregation machinery into a cytoskeletal
  system controlling cyanobacterial cell shape"
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2025'
...
