---
res:
  bibo_abstract:
  - Visualizing molecular localization at high resolution contributes to understanding
    of their functions and roles in physiological and pathological conditions. Sodium
    dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is a powerful
    electron microscopy method to study high-resolution two-dimensional distribution
    of transmembrane proteins and their tightly associated proteins on platinum-carbon
    replica. During treatment with SDS, unfixed proteins and intracellular organelle
    are dissolved and integral membrane proteins captured and stabilized by carbon
    and platinum deposition are denatured, retaining most of their antigenicity, and
    exposed on exoplasmic and protoplasmic surfaces of lipid monolayers. The exposure
    of these antigens on the surface of replica facilitates the accessibility of antibodies
    and therefore provides higher labeling efficiency than those obtained with other
    immunoelectron microscopy techniques. In this chapter, we describe the protocols
    of SDS-FRL adapted for mammalian brain samples and an additional procedure for
    fluorescence-guided electron microscopy for replica immunolabeling.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Harumi
      foaf_name: Harada, Harumi
      foaf_surname: Harada
      foaf_workInfoHomepage: http://www.librecat.org/personId=2E55CDF2-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7429-7896
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  bibo_doi: 10.1007/978-1-4939-3064-7_17
  dct_date: 2016^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0893-2336
  - http://id.crossref.org/issn/1940-6045
  - http://id.crossref.org/issn/9781493930630
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: High-Resolution Localization of Membrane Proteins by SDS-Digested Freeze-Fracture
    Replica Labeling (SDS-FRL)@
...