Cell-specific wiring routes information flow through hippocampal CA3

article Cell-specific wiring routes information flow through hippocampal CA3 published yes Jake Watson author 63836096-4690-11EA-BD4E-32803DDC885E0000-0002-8698-3823 Victor M Vargas Barroso author 2F55A9DE-F248-11E8-B48F-1D18A9856A87 Peter M Jonas author 353C1B58-F248-11E8-B48F-1D18A9856A870000-0001-5001-4804 PeJo department Biophysics and circuit function of a giant cortical glutamatergic synapse project Synaptic computations of the hippocampal CA3 circuitry project Mechanisms of GABA release in hippocampal circuits project ISTplus - Postdoctoral Fellowships project The hippocampus, critical for learning and memory, is dogmatically described as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an autoassociative network, and its PNs have diverse morphologies, intrinsic properties, and GC input levels. How PN subtypes compose this recurrent network is unknown. To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular patch-clamp recording and post hoc morphological analysis in mouse hippocampal slices. PNs can be divided into distinct “superficial” and “deep” subclasses, the latter including previously reported “athorny” cells. Subclasses have distinct input-output transformations and asymmetric connectivity, which is more abundant from superficial to deep PNs, splitting CA3 locally into two parallel recurrent networks. Coincident spontaneous inhibition occurs frequently within but not between subclasses, implying subclass-specific inhibitory innervation. Our results suggest two separately controlled sublayers for parallel information processing in hippocampal CA3. https://research-explorer.ista.ac.at/download/20099/20106/2025_CellReports_Watson.pdf application/pdfno Elsevier2025 eng Cell Reports 2639-1856 2211-1247 00154447230000210.1016/j.celrep.2025.116080 448 Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” Cell Reports. Elsevier, 2025. <a href="https://doi.org/10.1016/j.celrep.2025.116080">https://doi.org/10.1016/j.celrep.2025.116080</a>. Watson, J., Vargas Barroso, V. M., & Jonas, P. M. (2025). Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.116080">https://doi.org/10.1016/j.celrep.2025.116080</a> Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 44(8), 116080. J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025). J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes information flow through hippocampal CA3,” Cell Reports, vol. 44, no. 8. Elsevier, 2025. Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” Cell Reports, vol. 44, no. 8, 116080, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116080">10.1016/j.celrep.2025.116080</a>. Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 2025;44(8). doi:<a href="https://doi.org/10.1016/j.celrep.2025.116080">10.1016/j.celrep.2025.116080</a> 200992025-08-03T22:01:30Z2025-09-30T14:12:02Z