---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20187'
abstract:
- lang: eng
  text: Very long-chain fatty acids (VLCFAs), being constituents of different types
    of lipids, are critical factors in plant development, presumably due to their
    impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic
    reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase
    1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems
    (esm) mutant, a viable kcr1 allele presumably affecting surface properties of
    the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that
    impacts VLCFAs. The kcr1-2 plants show severe defects during different stages
    of development, which all correlate with defects in polar localization and subcellular
    trafficking of PIN auxin transporters and resulting asymmetric auxin distribution.
    Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests
    that KCR1 plays a role in delineating boundaries around meristematic and specialized
    differentiating tissues, including root and shoot meristems, initiating lateral
    roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced
    VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful
    tool to study VLCFA roles in plant development and highlights VLCFAs as critical
    developmental factors at the interface of cell polarity and tissue development.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy
  (especially Vanessa Zheden for technical assistance) and Life Science (in particular
  Dorota Jaworska) facilities at ISTA for their continuous support. Authors would
  like to thank Michelle Gallei for advice during the generation of the transgenic
  lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help
  and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and
  root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski
  for advice on abiotic stress experiments. We would like to thank Dolf Weijers for
  the SOSEKI (SOK) marker line seeds. This work has benefited from the support of
  IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian
  Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province
  (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant
  Sciences-SPS (ANR-17-EUR-0007)."
article_number: e70396
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
  full_name: Babic, David
  id: db566d23-f6e0-11ea-865d-e6f270e968e7
  last_name: Babic
- first_name: Rashed
  full_name: Abualia, Rashed
  id: 4827E134-F248-11E8-B48F-1D18A9856A87
  last_name: Abualia
  orcid: 0000-0002-9357-9415
- first_name: Lukas
  full_name: Fiedler, Lukas
  id: 7c417475-8972-11ed-ae7b-8b674ca26986
  last_name: Fiedler
- first_name: Linlin
  full_name: Qi, Linlin
  id: 44B04502-A9ED-11E9-B6FC-583AE6697425
  last_name: Qi
  orcid: 0000-0001-5187-8401
- first_name: Frédérique
  full_name: Tellier, Frédérique
  last_name: Tellier
- first_name: Adrijana
  full_name: Smoljan, Adrijana
  id: cced8a85-223e-11ed-af04-b0596c55053b
  last_name: Smoljan
- first_name: Hana
  full_name: Rakusova, Hana
  id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
  last_name: Rakusova
- first_name: Petr
  full_name: Valošek, Petr
  id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
  last_name: Valošek
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Jean Denis
  full_name: Faure, Jean Denis
  last_name: Faure
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty
    acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
    development. <i>Plant Journal</i>. 2025;123(3). doi:<a href="https://doi.org/10.1111/tpj.70396">10.1111/tpj.70396</a>
  apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml,
    J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis
    auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>.
    Wiley. <a href="https://doi.org/10.1111/tpj.70396">https://doi.org/10.1111/tpj.70396</a>
  chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier,
    Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty
    Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic
    Development.” <i>Plant Journal</i>. Wiley, 2025. <a href="https://doi.org/10.1111/tpj.70396">https://doi.org/10.1111/tpj.70396</a>.
  ieee: D. Babic <i>et al.</i>, “Biosynthesis of very long-chain fatty acids is required
    for Arabidopsis auxin-mediated embryonic and post-embryonic development,” <i>Plant
    Journal</i>, vol. 123, no. 3. Wiley, 2025.
  ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek
    P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain
    fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
    development. Plant Journal. 123(3), e70396.
  mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required
    for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant
    Journal</i>, vol. 123, no. 3, e70396, Wiley, 2025, doi:<a href="https://doi.org/10.1111/tpj.70396">10.1111/tpj.70396</a>.
  short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova,
    P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).
corr_author: '1'
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-10-12T22:30:13Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
- _id: GradSch
doi: 10.1111/tpj.70396
external_id:
  isi:
  - '001547884300001'
  pmid:
  - '40782342'
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fulldoi: https://doi.org/10.1111/tpj.70396
has_accepted_license: '1'
intvolume: '       123'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
  grant_number: I06123
  name: Peptide receptors for auxin canalization in Arabidopsis
publication: Plant Journal
publication_identifier:
  eissn:
  - 1365-313X
  issn:
  - 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
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    status: public
scopus_import: '1'
status: public
title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated
  embryonic and post-embryonic development
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 123
year: '2025'
...