---
res:
  bibo_abstract:
  - "Cortical development has been studied for many decades and with each passing
    year, we learn \r\nmore about the processes that govern the tightly regulated
    developmental program. Up until \r\nrecently, the field has struggled to gain
    reliable access to progenitors at the single-cell level, \r\nlimiting the resolution
    at which we can study cortical development in vivo. Therefore, much of \r\nthe
    data we have on mammalian cortical development is at the cell population level.
    The \r\nsubsequent pages of this thesis describe novel elements of cortical development
    in mice; \r\nacquired using a candidate gene approach in conjunction with Mosaic
    Analysis with Double \r\nMarkers (MADM) to achieve single cell resolution. In
    this thesis, I briefly provide an overview \r\nof the state of the field, describe
    the methodology used to quantify morphological \r\ncharacteristics of neurons
    and glia, and delve into the role of Phosphatase and tensin \r\nhomologue on chromosome
    ten (Pten) in neurogenesis and gliogenesis in the developing mouse \r\ncortex.
    By using MADM to generate genetic mosaics, in which only a small fraction of cells
    \r\nare mutated, to study population-level changes, we can bypass the early postnatal
    lethality \r\nreported in Pten conditional knockouts (cKOs) using Emx1-Cre. We
    used Emx1-CreERT2 to \r\ninduce clonal deletion of Pten, and quantify RGP neurogenic
    output at the single progenitor \r\nlevel. Pten-mutant neuron populations within
    the mosaics were significantly expanded. In the \r\nlatter parts of this thesis,
    we describe how Pten deletion leads to an increase in the abundance \r\nof astrocytes.
    We availed ourselves of the flexibility offered by the MADM system to generate
    \r\nPten mosaics within a series of distinct knockout tissues to test for the
    interactions between \r\nPten and a battery of genes involved in distinct pro-gliogenic
    signaling pathways. Our survey \r\nof Pten’s interactions with genes in key signaling
    pathways reveals that ERK1/2 are the critical \r\neffectors downstream of EGFR
    signaling essential for gliogenesis. Altogether, our results \r\ndemonstrate that
    Pten plays critical and distinct roles throughout cortical RGP lineage \r\nprogression.
    @eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Osvaldo
      foaf_name: Miranda, Osvaldo
      foaf_surname: Miranda
      foaf_workInfoHomepage: http://www.librecat.org/personId=862A3C56-A8BF-11E9-B4FA-D9E3E5697425
    orcid: 0000-0001-6618-6889
  bibo_doi: 10.15479/AT-ISTA-20212
  dct_date: 2025^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2663-337X
  - http://id.crossref.org/issn/978-3-99078-063-3
  dct_language: eng
  dct_publisher: Institute of Science and Technology Austria@
  dct_subject:
  - Pten
  - mtor
  - cortical development
  - MADM
  - Mapk
  dct_title: Unraveling the role of Pten in cortical stem cell lineage progression
    using MADM@
...