---
_id: '2022'
abstract:
- lang: eng
  text: Radial glial progenitors (RGPs) are responsible for producing nearly all neocortical
    neurons. To gain insight into the patterns of RGP division and neuron production,
    we quantitatively analyzed excitatory neuron genesis in the mouse neocortex using
    Mosaic Analysis with Double Markers, which provides single-cell resolution of
    progenitor division patterns and potential in vivo. We found that RGPs progress
    through a coherent program in which their proliferative potential diminishes in
    a predictable manner. Upon entry into the neurogenic phase, individual RGPs produce
    ∼8–9 neurons distributed in both deep and superficial layers, indicating a unitary
    output in neuronal production. Removal of OTX1, a transcription factor transiently
    expressed in RGPs, results in both deep- and superficial-layer neuron loss and
    a reduction in neuronal unit size. Moreover, ∼1/6 of neurogenic RGPs proceed to
    produce glia. These results suggest that progenitor behavior and histogenesis
    in the mammalian neocortex conform to a remarkably orderly and deterministic program.
article_processing_charge: No
author:
- first_name: Peng
  full_name: Gao, Peng
  last_name: Gao
- first_name: Maria P
  full_name: Postiglione, Maria P
  id: 2C67902A-F248-11E8-B48F-1D18A9856A87
  last_name: Postiglione
- first_name: Teresa
  full_name: Krieger, Teresa
  last_name: Krieger
- first_name: Luisirene
  full_name: Hernandez, Luisirene
  last_name: Hernandez
- first_name: Chao
  full_name: Wang, Chao
  last_name: Wang
- first_name: Zhi
  full_name: Han, Zhi
  last_name: Han
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Ryan
  full_name: Insolera, Ryan
  last_name: Insolera
- first_name: Kritika
  full_name: Chugh, Kritika
  last_name: Chugh
- first_name: Oren
  full_name: Kodish, Oren
  last_name: Kodish
- first_name: Kun
  full_name: Huang, Kun
  last_name: Huang
- first_name: Benjamin
  full_name: Simons, Benjamin
  last_name: Simons
- first_name: Liqun
  full_name: Luo, Liqun
  last_name: Luo
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Song
  full_name: Shi, Song
  last_name: Shi
citation:
  ama: Gao P, Postiglione MP, Krieger T, et al. Deterministic progenitor behavior
    and unitary production of neurons in the neocortex. <i>Cell</i>. 2014;159(4):775-788.
    doi:<a href="https://doi.org/10.1016/j.cell.2014.10.027">10.1016/j.cell.2014.10.027</a>
  apa: Gao, P., Postiglione, M. P., Krieger, T., Hernandez, L., Wang, C., Han, Z.,
    … Shi, S. (2014). Deterministic progenitor behavior and unitary production of
    neurons in the neocortex. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2014.10.027">https://doi.org/10.1016/j.cell.2014.10.027</a>
  chicago: Gao, Peng, Maria P Postiglione, Teresa Krieger, Luisirene Hernandez, Chao
    Wang, Zhi Han, Carmen Streicher, et al. “Deterministic Progenitor Behavior and
    Unitary Production of Neurons in the Neocortex.” <i>Cell</i>. Cell Press, 2014.
    <a href="https://doi.org/10.1016/j.cell.2014.10.027">https://doi.org/10.1016/j.cell.2014.10.027</a>.
  ieee: P. Gao <i>et al.</i>, “Deterministic progenitor behavior and unitary production
    of neurons in the neocortex,” <i>Cell</i>, vol. 159, no. 4. Cell Press, pp. 775–788,
    2014.
  ista: Gao P, Postiglione MP, Krieger T, Hernandez L, Wang C, Han Z, Streicher C,
    Papusheva E, Insolera R, Chugh K, Kodish O, Huang K, Simons B, Luo L, Hippenmeyer
    S, Shi S. 2014. Deterministic progenitor behavior and unitary production of neurons
    in the neocortex. Cell. 159(4), 775–788.
  mla: Gao, Peng, et al. “Deterministic Progenitor Behavior and Unitary Production
    of Neurons in the Neocortex.” <i>Cell</i>, vol. 159, no. 4, Cell Press, 2014,
    pp. 775–88, doi:<a href="https://doi.org/10.1016/j.cell.2014.10.027">10.1016/j.cell.2014.10.027</a>.
  short: P. Gao, M.P. Postiglione, T. Krieger, L. Hernandez, C. Wang, Z. Han, C. Streicher,
    E. Papusheva, R. Insolera, K. Chugh, O. Kodish, K. Huang, B. Simons, L. Luo, S.
    Hippenmeyer, S. Shi, Cell 159 (2014) 775–788.
corr_author: '1'
date_created: 2018-12-11T11:55:16Z
date_published: 2014-11-06T00:00:00Z
date_updated: 2025-09-29T11:57:49Z
day: '06'
ddc:
- '570'
department:
- _id: SiHi
- _id: Bio
doi: 10.1016/j.cell.2014.10.027
ec_funded: 1
external_id:
  isi:
  - '000344522000011'
file:
- access_level: open_access
  checksum: 6c5de8329bb2ffa71cba9fda750f14ce
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:08:47Z
  date_updated: 2020-07-14T12:45:25Z
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  file_name: IST-2016-423-v1+1_1-s2.0-S0092867414013154-main.pdf
  file_size: 4435787
  relation: main_file
file_date_updated: 2020-07-14T12:45:25Z
has_accepted_license: '1'
intvolume: '       159'
isi: 1
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
page: 775 - 788
project:
- _id: 25D61E48-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '618444'
  name: Molecular Mechanisms of Cerebral Cortex Development
- _id: 25D7962E-B435-11E9-9278-68D0E5697425
  grant_number: RGP0053/2014
  name: Quantitative Structure-Function Analysis of Cerebral Cortex Assembly at Clonal
    Level
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5050'
pubrep_id: '423'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Deterministic progenitor behavior and unitary production of neurons in the
  neocortex
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 159
year: '2014'
...