---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '20220'
abstract:
- lang: eng
text: Stress granules (SG) are biomolecular condensates that represent an adaptive
response of cells to various stresses, including heat. However, the cell type–specific
function and relevance of SG formation, especially during reproductive development,
are largely not understood. Here, we show that the meiotic A-type cyclin TARDY
ASYNCHRONOUS MEIOSIS (TAM) is recruited to SGs in male meiocytes of Arabidopsis
after exposure to heat. We find that the amino terminus of TAM is necessary and
sufficient for the localization of proteins to meiotic SGs. Swapping the amino
terminus of TAM with the one of its sister protein CYCA1;1 resulted in a separation-of-function
allele of TAM, which prevents the partitioning of TAM to SGs while restoring a
wild-type phenotype in a tam mutant background under nonheat stress conditions.
Notably, plants expressing this TAM version prematurely terminate meiosis under
heat resulting in unreduced gametes. Thus, the formation of TAM-containing SGs
is necessary for genome stability under heat stress.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We thank L. Strader (Duke University, Durham) and A. Holehouse (Washington
University, Saint Louis) for discussion and input in LLPS. We thank T. Nakagawa
(Shimane University, Matsue) for providing the pGWB604 Gateway vector containing
bar gene identified by Meiji Seika Kaisha Ltd. We thank M. Heese (Hamburg University)
for the critical reading and comments on this manuscript. We further thank J. Mehrmann
(Hamburg University) for technical assistance. We thank the ISTA imaging facility
for assistance for microscopy.\r\nThis project has received funding from JST-PRESTO
(JPMJPR18H7), JST-CREST (JPMJCR18H4), European Union’s Horizon 2020 under MSCA grant
101034413, and a federal grant from the state of Hamburg (LFF-BiCon)."
article_processing_charge: Yes
article_type: original
author:
- first_name: Joke G
full_name: De Jaeger-Braet, Joke G
id: 26bd38d3-c59a-11ee-a1af-d7a988cafcc5
last_name: De Jaeger-Braet
- first_name: Merle
full_name: Hartmann, Merle
last_name: Hartmann
- first_name: Lev
full_name: Böttger, Lev
last_name: Böttger
- first_name: Chao
full_name: Yang, Chao
id: 082e3e6e-8069-11ed-8390-c8cce7b1aaca
last_name: Yang
- first_name: Takahiro
full_name: Hamada, Takahiro
last_name: Hamada
- first_name: Stefan
full_name: Hoth, Stefan
last_name: Hoth
- first_name: Xiaoqi
full_name: Feng, Xiaoqi
id: e0164712-22ee-11ed-b12a-d80fcdf35958
last_name: Feng
orcid: 0000-0002-4008-1234
- first_name: Magdalena
full_name: Weingartner, Magdalena
last_name: Weingartner
- first_name: Arp
full_name: Schnittger, Arp
last_name: Schnittger
citation:
ama: De Jaeger-Braet JG, Hartmann M, Böttger L, et al. The recruitment of the A-type
cyclin TAM to stress granules is crucial for meiotic fidelity under heat. Science
Advances. 2025;11(32):eadr5694. doi:10.1126/sciadv.adr5694
apa: De Jaeger-Braet, J. G., Hartmann, M., Böttger, L., Yang, C., Hamada, T., Hoth,
S., … Schnittger, A. (2025). The recruitment of the A-type cyclin TAM to stress
granules is crucial for meiotic fidelity under heat. Science Advances.
AAAS. https://doi.org/10.1126/sciadv.adr5694
chicago: De Jaeger-Braet, Joke G, Merle Hartmann, Lev Böttger, Chao Yang, Takahiro
Hamada, Stefan Hoth, Xiaoqi Feng, Magdalena Weingartner, and Arp Schnittger. “The
Recruitment of the A-Type Cyclin TAM to Stress Granules Is Crucial for Meiotic
Fidelity under Heat.” Science Advances. AAAS, 2025. https://doi.org/10.1126/sciadv.adr5694.
ieee: J. G. De Jaeger-Braet et al., “The recruitment of the A-type cyclin
TAM to stress granules is crucial for meiotic fidelity under heat,” Science
Advances, vol. 11, no. 32. AAAS, p. eadr5694, 2025.
ista: De Jaeger-Braet JG, Hartmann M, Böttger L, Yang C, Hamada T, Hoth S, Feng
X, Weingartner M, Schnittger A. 2025. The recruitment of the A-type cyclin TAM
to stress granules is crucial for meiotic fidelity under heat. Science Advances.
11(32), eadr5694.
mla: De Jaeger-Braet, Joke G., et al. “The Recruitment of the A-Type Cyclin TAM
to Stress Granules Is Crucial for Meiotic Fidelity under Heat.” Science Advances,
vol. 11, no. 32, AAAS, 2025, p. eadr5694, doi:10.1126/sciadv.adr5694.
short: J.G. De Jaeger-Braet, M. Hartmann, L. Böttger, C. Yang, T. Hamada, S. Hoth,
X. Feng, M. Weingartner, A. Schnittger, Science Advances 11 (2025) eadr5694.
date_created: 2025-08-24T22:01:30Z
date_published: 2025-08-08T00:00:00Z
date_updated: 2025-09-30T14:24:10Z
day: '08'
ddc:
- '580'
department:
- _id: XiFe
doi: 10.1126/sciadv.adr5694
ec_funded: 1
external_id:
isi:
- '001549102600016'
file:
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creator: dernst
date_created: 2025-09-02T07:05:37Z
date_updated: 2025-09-02T07:05:37Z
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has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '32'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '08'
oa: 1
oa_version: Published Version
page: eadr5694
project:
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: The recruitment of the A-type cyclin TAM to stress granules is crucial for
meiotic fidelity under heat
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 11
year: '2025'
...