--- res: bibo_abstract: - 'The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Junko foaf_name: Toshima, Junko foaf_surname: Toshima - foaf_Person: foaf_givenName: Show foaf_name: Nishinoaki, Show foaf_surname: Nishinoaki - foaf_Person: foaf_givenName: Yoshifumi foaf_name: Sato, Yoshifumi foaf_surname: Sato - foaf_Person: foaf_givenName: Wataru foaf_name: Yamamoto, Wataru foaf_surname: Yamamoto - foaf_Person: foaf_givenName: Daiki foaf_name: Furukawa, Daiki foaf_surname: Furukawa - foaf_Person: foaf_givenName: Daria E foaf_name: Siekhaus, Daria E foaf_surname: Siekhaus foaf_workInfoHomepage: http://www.librecat.org/personId=3D224B9E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8323-8353 - foaf_Person: foaf_givenName: Akira foaf_name: Sawaguchi, Akira foaf_surname: Sawaguchi - foaf_Person: foaf_givenName: Jiro foaf_name: Toshima, Jiro foaf_surname: Toshima bibo_doi: 10.1038/ncomms4498 bibo_volume: 5 dct_date: 2014^xs_gYear dct_language: eng dct_publisher: Nature Publishing Group@ dct_title: Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole@ ...