{"pubrep_id":"616","publication_status":"published","language":[{"iso":"eng"}],"oa":1,"year":"2014","publisher":"Nature Publishing Group","volume":5,"day":"25","_id":"2024","publication":"Nature Communications","date_updated":"2021-01-12T06:54:48Z","article_number":"3498","file":[{"file_size":4803515,"date_created":"2018-12-12T10:11:11Z","content_type":"application/pdf","checksum":"614fb6579c86d1f95bdd95eeb9ab01b0","date_updated":"2020-07-14T12:45:25Z","file_name":"IST-2016-616-v1+1_DaSi_Bifurcation_Postprint.pdf","access_level":"open_access","creator":"system","relation":"main_file","file_id":"4864"}],"doi":"10.1038/ncomms4498","publist_id":"5048","ddc":["570"],"status":"public","title":"Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole","date_created":"2018-12-11T11:55:16Z","oa_version":"Submitted Version","file_date_updated":"2020-07-14T12:45:25Z","date_published":"2014-03-25T00:00:00Z","has_accepted_license":"1","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","type":"journal_article","month":"03","abstract":[{"text":"The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.","lang":"eng"}],"quality_controlled":"1","scopus_import":1,"department":[{"_id":"DaSi"}],"author":[{"full_name":"Toshima, Junko","last_name":"Toshima","first_name":"Junko"},{"full_name":"Nishinoaki, Show","last_name":"Nishinoaki","first_name":"Show"},{"first_name":"Yoshifumi","full_name":"Sato, Yoshifumi","last_name":"Sato"},{"full_name":"Yamamoto, Wataru","last_name":"Yamamoto","first_name":"Wataru"},{"first_name":"Daiki","full_name":"Furukawa, Daiki","last_name":"Furukawa"},{"first_name":"Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8323-8353","full_name":"Siekhaus, Daria E","last_name":"Siekhaus"},{"last_name":"Sawaguchi","full_name":"Sawaguchi, Akira","first_name":"Akira"},{"full_name":"Toshima, Jiro","last_name":"Toshima","first_name":"Jiro"}],"citation":{"chicago":"Toshima, Junko, Show Nishinoaki, Yoshifumi Sato, Wataru Yamamoto, Daiki Furukawa, Daria E Siekhaus, Akira Sawaguchi, and Jiro Toshima. “Bifurcation of the Endocytic Pathway into Rab5-Dependent and -Independent Transport to the Vacuole.” <i>Nature Communications</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncomms4498\">https://doi.org/10.1038/ncomms4498</a>.","apa":"Toshima, J., Nishinoaki, S., Sato, Y., Yamamoto, W., Furukawa, D., Siekhaus, D. E., … Toshima, J. (2014). Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms4498\">https://doi.org/10.1038/ncomms4498</a>","mla":"Toshima, Junko, et al. “Bifurcation of the Endocytic Pathway into Rab5-Dependent and -Independent Transport to the Vacuole.” <i>Nature Communications</i>, vol. 5, 3498, Nature Publishing Group, 2014, doi:<a href=\"https://doi.org/10.1038/ncomms4498\">10.1038/ncomms4498</a>.","ieee":"J. Toshima <i>et al.</i>, “Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole,” <i>Nature Communications</i>, vol. 5. Nature Publishing Group, 2014.","ista":"Toshima J, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus DE, Sawaguchi A, Toshima J. 2014. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications. 5, 3498.","ama":"Toshima J, Nishinoaki S, Sato Y, et al. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. <i>Nature Communications</i>. 2014;5. doi:<a href=\"https://doi.org/10.1038/ncomms4498\">10.1038/ncomms4498</a>","short":"J. Toshima, S. Nishinoaki, Y. Sato, W. Yamamoto, D. Furukawa, D.E. Siekhaus, A. Sawaguchi, J. Toshima, Nature Communications 5 (2014)."},"intvolume":"         5"}